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dc.contributor.authorRayaprolu, Vamseedhar
dc.contributor.authorRoyal, Perrine
dc.contributor.authorStengel, Karen
dc.contributor.authorSandoz, Guillaume
dc.contributor.authorKohout, Susy C.
dc.date.accessioned2018-10-21T15:23:12Z
dc.date.available2018-10-21T15:23:12Z
dc.date.issued2018-05
dc.identifier.citationRayaprolu, Vamseedhar, Perrine Royal, Karen Stengel, Guillaume Sandoz, and Susy C. Kohout. "Dimerization of the voltage-sensing phosphatase controls its voltage-sensing and catalytic activity." Journal of General Physiology 150, no. 5 (May 2018): 683-696. DOI:10.1085/jgp.201812064.en_US
dc.identifier.issn0022-1295
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14932
dc.description.abstractMultimerization is a key characteristic of most voltage-sensing proteins. The main exception was thought to be the Ciona intestinalis voltage-sensing phosphatase (Ci-VSP). In this study, we show that multimerization is also critical for Ci-VSP function. Using coimmunoprecipitation and single-molecule pull-down, we find that Ci-VSP stoichiometry is flexible. It exists as both monomers and dimers, with dimers favored at higher concentrations. We show strong dimerization via the voltage-sensing domain (VSD) and weak dimerization via the phosphatase domain. Using voltage-clamp fluorometry, we also find that VSDs cooperate to lower the voltage dependence of activation, thus favoring the activation of Ci-VSP. Finally, using activity assays, we find that dimerization alters Ci-VSP substrate specificity such that only dimeric Ci-VSP is able to dephosphorylate the 3-phosphate from PI(3,4,5)P3 or PI(3,4)P2 Our results indicate that dimerization plays a significant role in Ci-VSP function.en_US
dc.description.sponsorshipMontana State University; National Institutes of Health (R01GM111685, P20GM103474); Fondation pour la Recherche Médicale Equipe FRM (DEQ20170336753)en_US
dc.language.isoenen_US
dc.rightsCC BY-NC-SA: This license lets you remix, tweak, and build upon this work non-commercially, as long as you give credit to the original creator and license your new creations under the identical terms.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/legalcodeen_US
dc.titleDimerization of the voltage-sensing phosphatase controls its voltage-sensing and catalytic activityen_US
dc.typeArticleen_US
mus.citation.extentfirstpage683en_US
mus.citation.extentlastpage696en_US
mus.citation.issue5en_US
mus.citation.journaltitleJournal of General Physiologyen_US
mus.citation.volume150en_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1085/jgp.201812064en_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCell Biology & Neuroscience.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage5en_US
mus.contributor.orcidKohout, Susy C.|0000-0002-8159-8934en_US


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