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dc.contributor.authorAmenabar, Maximiliano J.
dc.contributor.authorColman, Daniel R.
dc.contributor.authorPoudel, Saroj
dc.contributor.authorRoden, Eric E.
dc.contributor.authorBoyd, Eric S.
dc.identifier.citationAmenabar, Maximiliano, Daniel R. Colman, Saroj Poudel, Eric E. Roden, and Eric S. Boyd. "Electron Acceptor Availability Alters Carbon and Energy Metabolism in a Thermoacidophile." Environmental Microbiology 20, no. 7 (May 2018): 2523-2537. DOI:10.1111/1462-2920.14270.en_US
dc.description.abstractThe thermoacidophilic Acidianus strain DS80 displays versatility in its energy metabolism and can grow autotrophically and heterotrophically with elemental sulfur (S°), ferric iron (Fe3+) or oxygen (O2) as electron acceptors. Here, we show that autotrophic and heterotrophic growth with S° as the electron acceptor is obligately dependent on hydrogen (H2) as electron donor; organic substrates such as acetate can only serve as a carbon source. In contrast, organic substrates such as acetate can serve as electron donor and carbon source for Fe3+ or O2 grown cells. During growth on S° or Fe3+ with H2 as an electron donor, the amount of CO2 assimilated into biomass decreased when cultures were provided with acetate. The addition of CO2 to cultures decreased the amount of acetate mineralized and assimilated and increased cell production in H2/Fe3+ grown cells but had no effect on H2/S° grown cells. In acetate/Fe3+ grown cells, the presence of H2 decreased the amount of acetate mineralized as CO2 in cultures compared to those without H2. These results indicate that electron acceptor availability constrains the variety of carbon sources used by this strain. Addition of H2 to cultures overcomes this limitation and alters heterotrophic metabolism.en_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).en_US
dc.titleElectron Acceptor Availability Alters Carbon and Energy Metabolism in a Thermoacidophileen_US
mus.citation.journaltitleEnvironmental Microbiologyen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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