Show simple item record

dc.contributor.authorFang, Fang
dc.contributor.authorAngulo, Benjamin
dc.contributor.authorXia, Ninuo
dc.contributor.authorSukhwani, Meena
dc.contributor.authorWang, Zhengyuan
dc.contributor.authorCarey, Charles C.
dc.contributor.authorMazurie, Aurélien J.
dc.contributor.authorCui, Jun
dc.contributor.authorWilkinson, Royce A.
dc.contributor.authorWiedenheft, Blake A.
dc.contributor.authorIrie, Naoko
dc.contributor.authorSurani, M. Azim
dc.contributor.authorOrwig, Kyle E.
dc.contributor.authorReijo Pera, Renee A.
dc.date.accessioned2018-11-01T15:34:11Z
dc.date.available2018-11-01T15:34:11Z
dc.date.issued2018-04
dc.identifier.citationFang, Fang, Benjamin Angulo, Ninuo Xia, Meena Sukhwani, Zhengyuan Wang, Charles C. Carey, Aurélien J. Mazurie, Jun Cui, Royce Wilkinson, Blake Wiedenheft, Naoko Irie, M. Azim Surani, Kyle E. Orwig, and Renee A. Reijo Pera. "A PAX5-OCT4-PRDM1 developmental switch specifies human primordial germ cells." Nature Cell Biology 20, no. 6 (April 2018): 655-665. DOI:10.1038/s41556-018-0094-3.en_US
dc.identifier.issn1476-4679
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14977
dc.description.abstractDysregulation of genetic pathways during human germ cell development leads to infertility. Here, we analysed bona fide human primordial germ cells (hPGCs) to probe the developmental genetics of human germ cell specification and differentiation. We examined the distribution of OCT4 occupancy in hPGCs relative to human embryonic stem cells (hESCs). We demonstrated that development, from pluripotent stem cells to germ cells, is driven by switching partners with OCT4 from SOX2 to PAX5 and PRDM1. Gain- and loss-of-function studies revealed that PAX5 encodes a critical regulator of hPGC development. Moreover, an epistasis analysis indicated that PAX5 acts upstream of OCT4 and PRDM1. The PAX5-OCT4-PRDM1 proteins form a core transcriptional network that activates germline and represses somatic programmes during human germ cell differentiation. These findings illustrate the power of combined genome editing, cell differentiation and engraftment for probing human developmental genetics that have historically been difficult to study.en_US
dc.language.isoenen_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en_US
dc.titleA PAX5-OCT4-PRDM1 developmental switch specifies human primordial germ cellsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage655en_US
mus.citation.extentlastpage665en_US
mus.citation.issue6en_US
mus.citation.journaltitleNature Cell Biologyen_US
mus.citation.volume20en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1038/s41556-018-0094-3en_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCell Biology & Neuroscience.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage9en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record