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dc.contributor.authorGoffena, Joy
dc.contributor.authorLefcort, Frances
dc.contributor.authorZhang, Yongqing
dc.contributor.authorLehrmann, Elin
dc.contributor.authorChaverra, Marta
dc.contributor.authorFelig, Jehremy
dc.contributor.authorWalters, Joseph
dc.contributor.authorBuksch, Richard
dc.contributor.authorBecker, Kevin G.
dc.contributor.authorGeorge, Lynn
dc.date.accessioned2018-11-01T15:56:09Z
dc.date.available2018-11-01T15:56:09Z
dc.date.issued2018-03
dc.identifier.citationGoffena, Joy, Frances Lefcort, Yongqing Zhang, Elin Lehrmann, Marta Chaverra, Jehremy Felig, Joseph Walters, Richard Buksch, Kevin G Becker, and Lynn George. "Elongator and codon bias regulate protein levels in mammalian peripheral neurons." Nature Communications 9 (March 2018): 1-10. DOI:10.1038/s41467-018-03221-z.en_US
dc.identifier.issn2041-1723
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14989
dc.description.abstractFamilial dysautonomia (FD) results from mutation in IKBKAP/ELP1, a gene encoding the scaffolding protein for the Elongator complex. This highly conserved complex is required for the translation of codon-biased genes in lower organisms. Here we investigate whether Elongator serves a similar function in mammalian peripheral neurons, the population devastated in FD. Using codon-biased eGFP sensors, and multiplexing of codon usage with transcriptome and proteome analyses of over 6,000 genes, we identify two categories of genes, as well as specific gene identities that depend on Elongator for normal expression. Moreover, we show that multiple genes in the DNA damage repair pathway are codon-biased, and that with Elongator loss, their misregulation is correlated with elevated levels of DNA damage. These findings link Elongator's function in the translation of codon-biased genes with both the developmental and neurodegenerative phenotypes of FD, and also clarify the increased risk of cancer associated with the disease.en_US
dc.description.sponsorshipNational Institutes of Health (NIH), R15NS090384; National Institute of General Medical Sciences of the NIH, P20GM103474; Intramural Research Program of the NIH, National Institute on Aging; NIH R01NS086796en_US
dc.language.isoenen_US
dc.rightsCC BY 4.0, This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcodeen_US
dc.titleElongator and codon bias regulate protein levels in mammalian peripheral neuronsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage10en_US
mus.citation.journaltitleNature Communicationsen_US
mus.citation.volume9en_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.doi10.1038/s41467-018-03221-zen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentMicrobiology & Cell Biology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage5en_US


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CC BY 4.0, This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.
Except where otherwise noted, this item's license is described as CC BY 4.0, This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.

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