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dc.contributor.authorYan, Dong-Hui
dc.contributor.authorSong, Xiaoyu
dc.contributor.authorLi, Hongchang
dc.contributor.authorLuo, Tushou
dc.contributor.authorDou, Guiming
dc.contributor.authorStrobel, Gary A.
dc.date.accessioned2018-11-14T17:36:55Z
dc.date.available2018-11-14T17:36:55Z
dc.date.issued2018-05
dc.identifier.citationYan, Dong-Hui, Xiaoyu Song, Hongchang Li, Tushou Luo, Guiming Dou, and Gary Strobel. "Antifungal Activities of Volatile Secondary Metabolites of Four Diaporthe Strains Isolated from Catharanthus roseus." Journal of Fungi 4, no. 2 (May 2018). DOI:10.3390/jof4020065.en_US
dc.identifier.issn2309-608X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14999
dc.description.abstractFour endophytic fungi were isolated from the medicinal plant, Catharanthus roseus, and were identified as Diaporthe spp. with partial translation elongation factor 1-alpha (TEF1), beta-tubulin (TUB), histone H3 (HIS), calmodulin (CAL) genes, and rDNA internal transcribed spacer (ITS) region (TEF1-TUB-HIS--CAL-ITS) multigene phylogeny suggested for species delimitation in the Diaporthe genus. Each fungus produces a unique mixture of volatile organic compounds (VOCs) with an abundant mixture of terpenoids analyzed by headspace solid-phase microextraction (SPME) fiber-GC/MS. These tentatively-detected terpenes included α-muurolene, β-phellandrene, γ-terpinene, and α-thujene, as well as other minor terpenoids, including caryophyllene, patchoulene, cedrene, 2-carene, and thujone. The volatile metabolites of each isolate showed antifungal properties against a wide range of plant pathogenic test fungi and oomycetes, including Alternaria alternata, Botrytis cinerea, Colletotrichum gloeosporioides, Fusarium graminearum, and Phytophthora cinnamomi. The growth inhibition of the pathogens varied between 10% and 60% within 72 h of exposure. To our knowledge, the endophytic Diaporthe-like strains are first reported from Catharanthus roseus. VOCs produced by each strain of the endophytic Diaporthe fungi were unique components with dominant monoterpenes comparing to known Diaporthe fungal VOCs. A discussion is presented on the inhibitive bioactivities of secondary metabolites among endophytic Diaporthe fungi and this medicinal plant.en_US
dc.description.sponsorshipFundamental Research Funds of CAF (CAFYBB2017MA010)en_US
dc.language.isoenen_US
dc.rightsCC BY, This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcodeen_US
dc.titleAntifungal Activities of Volatile Secondary Metabolites of Four Diaporthe Strains Isolated from Catharanthus roseusen_US
dc.typeArticleen_US
mus.citation.issue2en_US
mus.citation.journaltitleJournal of Fungien_US
mus.citation.volume4en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.3390/jof4020065en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage13en_US


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CC BY, This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.
Except where otherwise noted, this item's license is described as CC BY, This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.