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dc.contributor.authorOzseyhan, Mehmet E.
dc.contributor.authorLi, Pengcheng
dc.contributor.authorNa, GunNam
dc.contributor.authorLi, Zhenjing
dc.contributor.authorWang, Changlu
dc.contributor.authorLu, Chaofu
dc.date.accessioned2018-12-04T16:38:11Z
dc.date.available2018-12-04T16:38:11Z
dc.date.issued2018-09
dc.identifier.citationOzseyhan, Mehmet, Pengcheng Li, GunNam Na, Zhenjing Li, Changlu Wang, and Chaofu Lu. "Improved fatty acid profiles in seeds of Camelina sativa by artificial microRNA mediated FATB gene suppression." Biochemical & Biophysical Research Communications 503, no. 2 (September 2018): 621-624. DOI:10.1016/j.bbrc.2018.06.051.en_US
dc.identifier.issn0006-291X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/15034
dc.description.abstractThe fatty acid profile of plant oils determines their quality and uses. Saturated fatty acids are often not desirable from the standpoints of nutrition and some industrial applications. Camelina sativa is a re-emerged oilseed crop, however its oil needs to be improved to meet different application requirements. In this study, saturated fatty acids were greatly reduced by down-regulating genes encoding the fatty acyl-ACP thioesterases (FATB). An artificial microRNA (amiFATB) was created by replacing a microRNA sequence in the camelina Csa-miR159a gene with a FATB gene specific sequence. Seed-specific expression of amiFATB caused a 45% reduction of palmitic acid (16:0) and a 38% reduction of stearic acid (18:0) compared to wildtype seeds. The total saturated fatty acid content was decreased by 35% from 14.6% to 9.4% of total fatty acids. When amiFATB was expressed in a high-oleic acid transgenic line, it caused further increased oleic acid content. This work demonstrates that the FATB genes in camelina can be effectively knocked down by an artificial microRNA targeting gene-specific sequences, thus provides an additional tool to improve seed oils for desired properties.en_US
dc.description.sponsorshipUS Department of Energy (DE-SC0016400); National Science Foundation (DBI-1339358); US Department of Agriculture (1015638)en_US
dc.language.isoenen_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en_US
dc.titleImproved fatty acid profiles in seeds of Camelina sativa by artificial microRNA mediated FATB gene suppressionen_US
dc.typeArticleen_US
mus.citation.extentfirstpage621en_US
mus.citation.extentlastpage624en_US
mus.citation.issue2en_US
mus.citation.journaltitleBiochemical & Biophysical Research Communicationsen_US
mus.citation.volume503en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1016/j.bbrc.2018.06.051en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage4en_US
mus.contributor.orcidLu, Chaofu|0000-0001-6901-7490en_US


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