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dc.contributor.authorFixen, Kathryn R.
dc.contributor.authorPal Chowdhury, Nilanjan
dc.contributor.authorMartinez-Perez, Marta
dc.contributor.authorPoudel, Saroj
dc.contributor.authorBoyd, Eric S.
dc.contributor.authorHarwood, Caroline S.
dc.identifier.citationFixen, Kathryn R. , Nilanjan Pal Chowdhury, Marta Martinez-Perez, Saroj Poudel, Eric S. Boyd, and Caroline S. Harwood. "The path of electron transfer to nitrogenase in a phototrophic alpha-proteobacterium." Environmental Microbiology 20, no. 7 (July 2018): 2500-2508. DOI:10.1111/1462-2920.14262.en_US
dc.description.abstractThe phototrophic alpha-proteobacterium, Rhodopseudomonas palustris, is a model for studies of regulatory and physiological parameters that control the activity of nitrogenase. This enzyme produces the energy-rich compound H2 , in addition to converting N2 gas to NH3 . Nitrogenase is an ATP-requiring enzyme that uses large amounts of reducing power, but the electron transfer pathway to nitrogenase in R. palustris was incompletely known. Here, we show that the ferredoxin, Fer1, is the primary but not sole electron carrier protein encoded by R. palustris that serves as an electron donor to nitrogenase. A flavodoxin, FldA, is also an important electron donor, especially under iron limitation. We present a model where the electron bifurcating complex, FixABCX, can reduce both ferredoxin and flavodoxin to transfer electrons to nitrogenase, and we present bioinformatic evidence that FixABCX and Fer1 form a conserved electron transfer pathway to nitrogenase in nitrogen-fixing proteobacteria. These results may be useful in the design of strategies to reroute electrons generated during metabolism of organic compounds to nitrogenase to achieve maximal activity.en_US
dc.description.sponsorshipU.S. Department of Energy (DE‐SC0012518)en_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).en_US
dc.titleThe path of electron transfer to nitrogenase in a phototrophic alpha-proteobacteriumen_US
mus.citation.journaltitleEnvironmental Microbiologyen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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