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dc.contributor.authorChowdhury, Saikat
dc.contributor.authorCarter, Joshua
dc.contributor.authorRollins, MaryClare F.
dc.contributor.authorJackson, Ryan N.
dc.contributor.authorHoffmann, Connor
dc.contributor.authorNosaka, Lyn’Al
dc.contributor.authorBondy-Denomy, Joseph
dc.contributor.authorMaxwell, Karen L.
dc.contributor.authorDavidson, Alan R.
dc.contributor.authorFischer, Elizabeth R.
dc.contributor.authorLander, Gabriel C.
dc.contributor.authorWiedenheft, Blake A.
dc.date.accessioned2019-01-25T16:16:58Z
dc.date.available2019-01-25T16:16:58Z
dc.date.issued2017-03
dc.identifier.citationChowdhury, Saikat, Joshua Carter, MaryClare F. Rollins, Sarah M. Golden, Ryan N. Jackson, Connor Hoffmann, Lyn'AI Nosaka, Joseph Bondy-Denomy, Karen L. Maxwell, Alan R. Davidson, Elizabeth R. Fischer, Gabriel C. Lander, and Blake Wiedenheft. "Structure reveals mechanisms of viral suppressors that intercept a CRISPR RNA-guided surveillance complex." Cell 169, no. 1 (March 2017): 47-57. doi: 10.1016/j.cell.2017.03.012.en_US
dc.identifier.issn0092-8674
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/15158
dc.description.abstractGenetic conflict between viruses and their hosts drives evolution and genetic innovation. Prokaryotes evolved CRISPR-mediated adaptive immune systems for protection from viral infection, and viruses have evolved diverse anti-CRISPR (Acr) proteins that subvert these immune systems. The adaptive immune system in Pseudomonas aeruginosa (type I-F) relies on a 350 kDa CRISPR RNA (crRNA)-guided surveillance complex (Csy complex) to bind foreign DNA and recruit a trans-acting nuclease for target degradation. Here, we report the cryo-electron microscopy (cryo-EM) structure of the Csy complex bound to two different Acr proteins, AcrF1 and AcrF2, at an average resolution of 3.4 Å. The structure explains the molecular mechanism for immune system suppression, and structure-guided mutations show that the Acr proteins bind to residues essential for crRNA-mediated detection of DNA. Collectively, these data provide a snapshot of an ongoing molecular arms race between viral suppressors and the immune system they target.en_US
dc.description.sponsorshipNational Institutes of Health (P20GM103500, P30GM110732-03, R01GM110270, and R01GM108888); National Science Foundation (EPS-110134); National Institutes of Health (F32 GM108436, DP2EB020402);en_US
dc.language.isoenen_US
dc.rightsCC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/legalcodeen_US
dc.titleStructure reveals mechanisms of viral suppressors that intercept a CRISPR RNA-guided surveillance complexen_US
dc.typeArticleen_US
mus.citation.extentfirstpage47en_US
mus.citation.extentlastpage57en_US
mus.citation.issue1en_US
mus.citation.journaltitleCellen_US
mus.citation.volume169en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1016/j.cell.2017.03.012en_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage3en_US
mus.contributor.orcidCarter, Joshua|0000-0002-9194-7966en_US
mus.contributor.orcidJackson, Ryan N.|0000-0001-7403-3794en_US


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CC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.
Except where otherwise noted, this item's license is described as CC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.

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