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dc.contributor.authorSalinas, Daniel
dc.contributor.authorMumey, Brendan M.
dc.contributor.authorJune, Ronald K.
dc.date.accessioned2019-02-26T18:24:08Z
dc.date.available2019-02-26T18:24:08Z
dc.date.issued2018-02
dc.identifier.citationSalinas, Daniel, Brendan M. Mumey, and Ronald K. June. "Physiological dynamic compression regulates central energy metabolism in primary human chondrocytes." Biomechanics and Modeling in Mechanobiology 18 (February 2019): 69-77. DOI:10.1007/s10237-018-1068-x.en_US
dc.identifier.issn1617-7959
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/15297
dc.description.abstractChondrocytes use the pathways of central metabolism to synthesize molecular building blocks and energy for cartilage homeostasis. An interesting feature of the in vivo chondrocyte environment is the cyclical loading generated in various activities (e.g., walking). However, it is unknown whether central metabolism is altered by mechanical loading. We hypothesized that physiological dynamic compression alters central metabolism in chondrocytes to promote production of amino acid precursors for matrix synthesis. We measured the expression of central metabolites (e.g., glucose, its derivatives, and relevant co-factors) for primary human osteoarthritic chondrocytes in response to 0–30 minutes of compression. To analyze the data, we used principal components analysis and ANOVA-simultaneous components analysis, as well as metabolic flux analysis. Compression-induced metabolic responses consistent with our hypothesis. Additionally, these data show that chondrocyte samples from different patient donors exhibit different sensitivity to compression. Most importantly, we find that grade IV osteoarthritic chondrocytes are capable of synthesizing non-essential amino acids and precursors in response to mechanical loading. These results suggest that further advances in metabolic engineering of chondrocyte mechanotransduction may yield novel translational strategies for cartilage repair.en_US
dc.description.sponsorshipNational Science Foundation (1342420, 1554708 and 1542262); National Institutes of Health (P20GM103474)en_US
dc.language.isoenen_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en_US
dc.titlePhysiological dynamic compression regulates central energy metabolism in primary human chondrocytesen_US
dc.typeArticleen_US
mus.citation.extentfirstpage69en_US
mus.citation.extentlastpage77en_US
mus.citation.issue1en_US
mus.citation.journaltitleBiomechanics and Modeling in Mechanobiologyen_US
mus.citation.volume18en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1007/s10237-018-1068-xen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentComputer Science.en_US
mus.relation.departmentMechanical & Industrial Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage16en_US


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