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dc.contributor.authorMaaty, Walid S.
dc.contributor.authorWiedenheft, Blake A.
dc.contributor.authorTarlykov, Pavel V.
dc.contributor.authorSchaff, Nathan
dc.contributor.authorHeinemann, Joshua V.
dc.contributor.authorRobison-Cox, James
dc.contributor.authorDougherty, Amanda
dc.contributor.authorBlum, Paul
dc.contributor.authorLawrence, C. Martin
dc.contributor.authorDouglas, Trevor
dc.contributor.authorYoung, Mark J.
dc.contributor.authorBothner, Brian
dc.date.accessioned2019-04-16T14:30:49Z
dc.date.available2019-04-16T14:30:49Z
dc.date.issued2009-09
dc.identifier.citationMaaty, Walid S., Blake A. Wiedenheft, Pavel V. Tarlykov, Nathan Schaff, Joshua V. Heinemann, James Robison-Cox, Jacob J. Valenzuela, et al. “Something Old, Something New, Something Borrowed; How the Thermoacidophilic Archaeon Sulfolobus Solfataricus Responds to Oxidative Stress.” PLoS ONE 4, no. 9 (September 16, 2009): e6964. doi:10.1371/journal.pone.0006964.en_US
dc.identifier.issn1932-6203
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/15428
dc.description.abstractTo avoid molecular damage of biomolecules due to oxidation, all cells have evolved constitutive and responsive systems to mitigate and repair chemical modifications. Archaea have adapted to some of the most extreme environments known to support life, including highly oxidizing conditions. However, in comparison to bacteria and eukaryotes, relatively little is known about the biology and biochemistry of archaea in response to changing conditions and repair of oxidative damage. In this study transcriptome, proteome, and chemical reactivity analyses of hydrogen peroxide (H2O2) induced oxidative stress in Sulfolobus solfataricus (P2) were conducted. Microarray analysis of mRNA expression showed that 102 transcripts were regulated by at least 1.5 fold, 30 minutes after exposure to 30 µM H2O2. Parallel proteomic analyses using two-dimensional differential gel electrophoresis (2D-DIGE), monitored more than 800 proteins 30 and 105 minutes after exposure and found that 18 had significant changes in abundance. A recently characterized ferritin-like antioxidant protein, DPSL, was the most highly regulated species of mRNA and protein, in addition to being post-translationally modified. As expected, a number of antioxidant related mRNAs and proteins were differentially regulated. Three of these, DPSL, superoxide dismutase, and peroxiredoxin were shown to interact and likely form a novel supramolecular complex for mitigating oxidative damage. A scheme for the ability of this complex to perform multi-step reactions is presented. Despite the central role played by DPSL, cells maintained a lower level of protection after disruption of the dpsl gene, indicating a level of redundancy in the oxidative stress pathways of S. solfataricus. This work provides the first “omics” scale assessment of the oxidative stress response for an archeal organism and together with a network analysis using data from previous studies on bacteria and eukaryotes reveals evolutionarily conserved pathways where complex and overlapping defense mechanisms protect against oxygen toxicity.en_US
dc.description.sponsorshipNational Center For Research Resources COBRE Program grants NSF-MCB 0646499, NIH P20 RR-020185; NSF grant MCB-0235167en_US
dc.language.isoenen_US
dc.rightsCC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/legalcodeen_US
dc.titleSomething old, something new, something borrowed; how the thermoacidophilic archaeon Sulfolobus solfataricus responds to oxidative stressen_US
dc.typeArticleen_US
mus.citation.issue9en_US
mus.citation.journaltitlePLoS Oneen_US
mus.citation.volume4en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1371/journal.pone.0006964en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentMathematical Sciences.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupMT INBRE Bioinformatics and Biostatistics Core.en_US
mus.relation.researchgroupThermal Biology Institute (TBI).en_US
mus.data.thumbpage13en_US
mus.contributor.orcidBothner, Brian|0000-0003-1295-9609en_US


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CC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.
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