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dc.contributor.authorBehnke, Michael S.
dc.contributor.authorWootton, John C.
dc.contributor.authorLehmann, Margaret M.
dc.contributor.authorRadke, Josh B.
dc.contributor.authorLucas, Olivier
dc.contributor.authorNawas, Julie
dc.contributor.authorSibley, L. David
dc.contributor.authorWhite, Michael W.
dc.date.accessioned2019-04-17T20:56:24Z
dc.date.available2019-04-17T20:56:24Z
dc.date.issued2010-08
dc.identifier.citationBehnke, Michael S., John C. Wootton, Margaret M. Lehmann, Josh B. Radke, Olivier Lucas, Julie Nawas, L. David Sibley, and Michael W. White. “Coordinated Progression through Two Subtranscriptomes Underlies the Tachyzoite Cycle of Toxoplasma Gondii.” Edited by Gordon Langsley. PLoS ONE 5, no. 8 (August 26, 2010): e12354. doi:10.1371/journal.pone.0012354.en_US
dc.identifier.issn1932-6203
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/15438
dc.description.abstractBackground Apicomplexan parasites replicate by varied and unusual processes where the typically eukaryotic expansion of cellular components and chromosome cycle are coordinated with the biosynthesis of parasite-specific structures essential for transmission. Methodology/Principal Findings Here we describe the global cell cycle transcriptome of the tachyzoite stage of Toxoplasma gondii. In dividing tachyzoites, more than a third of the mRNAs exhibit significant cyclical profiles whose timing correlates with biosynthetic events that unfold during daughter parasite formation. These 2,833 mRNAs have a bimodal organization with peak expression occurring in one of two transcriptional waves that are bounded by the transition into S phase and cell cycle exit following cytokinesis. The G1-subtranscriptome is enriched for genes required for basal biosynthetic and metabolic functions, similar to most eukaryotes, while the S/M-subtranscriptome is characterized by the uniquely apicomplexan requirements of parasite maturation, development of specialized organelles, and egress of infectious daughter cells. Two dozen AP2 transcription factors form a series through the tachyzoite cycle with successive sharp peaks of protein expression in the same timeframes as their mRNA patterns, indicating that the mechanisms responsible for the timing of protein delivery might be mediated by AP2 domains with different promoter recognition specificities. Conclusion/Significance Underlying each of the major events in apicomplexan cell cycles, and many more subordinate actions, are dynamic changes in parasite gene expression. The mechanisms responsible for cyclical gene expression timing are likely crucial to the efficiency of parasite replication and may provide new avenues for interfering with parasite growth.en_US
dc.description.sponsorshipNational Institutes of Health grants AI 48390, AI077662, AI072739, AI034036; Intramural program of NCBI; National Library of Medicineen_US
dc.language.isoenen_US
dc.rightsCC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/legalcodeen_US
dc.titleCoordinated progression through two subtranscriptomes underlies the tachyzoitecycle of Toxoplasma gondiien_US
dc.typeArticleen_US
mus.citation.issue8en_US
mus.citation.journaltitlePLoS Oneen_US
mus.citation.volume5en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1371/journal.pone.0012354en_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupMT INBRE Bioinformatics and Biostatistics Core.en_US
mus.data.thumbpage13en_US


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CC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.
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