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dc.contributor.authorSidar, Barkan
dc.contributor.authorJenkins, Brittany R.
dc.contributor.authorHuang, Sha
dc.contributor.authorSpence, Jason R.
dc.contributor.authorWalk, Seth T.
dc.date.accessioned2020-04-20T20:25:02Z
dc.date.available2020-04-20T20:25:02Z
dc.date.issued2019-09
dc.identifier.citationSidar, Barkan, Brittany R. Jenkins, Sha Huang, Jason R. Spence, Seth T. Walk, and James N. Wilking. “Long-Term Flow through Human Intestinal Organoids with the Gut Organoid Flow Chip (GOFlowChip).” Lab on a Chip 19, no. 20 (2019): 3552–3562. doi:10.1039/c9lc00653b.en_US
dc.identifier.issn1473-0197
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/15847
dc.description.abstractHuman intestinal organoids (HIOs) are millimeter-scale models of the human intestinal epithelium and hold tremendous potential for advancing fundamental and applied biomedical research. HIOs resemble the native gut in that they consist of a fluid-filled lumen surrounded by a polarized epithelium and associated mesenchyme; however, their topologically-closed, spherical shape prevents flow through the interior luminal space, making the system less physiological and leading to the buildup of cellular and metabolic waste. These factors ultimately limit experimentation inside the HIOs. Here, we present a millifluidic device called the gut organoid flow chip (GOFlowChip), which we use to “port” HIOs and establish steady-state liquid flow through the lumen for multiple days. This long-term flow is enabled by the use of laser-cut silicone gaskets, which allow liquid in the device to be slightly pressurized, suppressing bubble formation. To demonstrate the utility of the device, we establish separate luminal and extraluminal flow and use luminal flow to remove accumulated waste. This represents the first demonstration of established liquid flow through the luminal space of a gastrointestinal organoid over physiologically relevant time scales. Flow cytometry results reveal that HIO cell viability is unaffected by long-term porting and luminal flow. We expect the real-time, long-term control over luminal and extraluminal contents provided by the GOFlowChip will enable a wide variety of studies including intestinal secretion, absorption, transport, and co-culture with intestinal microorganisms.en_US
dc.rightsCC BY-NC: This license lets you remix, tweak, and build upon this work non-commercially, and although your new works must also acknowledge the original creator and be non-commercial, you don’t have to license your derivative works on the same terms.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/legalcodeen_US
dc.titleLong-Term Flow through Human Intestinal Organoids with the Gut Organoid Flow Chip (GOFlowChip)en_US
dc.typeArticleen_US
mus.citation.extentfirstpage3552en_US
mus.citation.extentlastpage3562en_US
mus.citation.issue20en_US
mus.citation.journaltitleLab on a Chipen_US
mus.citation.volume19en_US
mus.identifier.doi10.1039/c9lc00653ben_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US


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CC BY-NC: This license lets you remix, tweak, and build upon this work non-commercially, and although your new works must also acknowledge the original creator and be non-commercial, you don’t have to license your derivative works on the same terms.
Except where otherwise noted, this item's license is described as CC BY-NC: This license lets you remix, tweak, and build upon this work non-commercially, and although your new works must also acknowledge the original creator and be non-commercial, you don’t have to license your derivative works on the same terms.