Type I-F CRISPR-Cas provides protection from DNA, but not RNA phages
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Date
2019-10
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Abstract
The type I-F CRISPR–Cas system from Pseudomonas aeruginosa (PA14) provides sequence-specific elimination of invading DNA1,2. Recently, a paper published in Cell Research reported that this system targets mRNA through a non-canonical mechanism3. Here, we implement the proposed design rules for generating CRISPR-RNA (crRNA)-guides that target RNA and test whether the proposed RNA-targeting activity provides immunity against RNA phage infection. Our experiments reveal that the type I-F CRISPR system provides protection from DNA, but not RNA phages.
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Buyukyoruk, Murat, and Blake Wiedenheft. “Type I-F CRISPR–Cas Provides Protection from DNA, but Not RNA Phages.” Cell Discovery 5, no. 1 (October 22, 2019). doi:10.1038/s41421-019-0123-9.