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dc.contributor.authorDe Grazia, Antonio
dc.contributor.authorLuTheryn, Gareth
dc.contributor.authorMeghdadi, Alireza
dc.contributor.authorMosayyebi, Ali
dc.contributor.authorEspinosa-Ortiz, Erika J.
dc.contributor.authorGerlach, Robin
dc.contributor.authorCarugo, Dario
dc.identifier.citationDe Grazia, Antonio, Gareth LuTheryn, Alireza Meghdadi, Ali Mosayyebi, Erika Espinosa-Ortiz, Robin Gerlach, and Dario Carugo. “A Microfluidic-Based Investigation of Bacterial Attachment in Ureteral Stents.” Micromachines 11, no. 4 (April 13, 2020): 408. doi:10.3390/mi11040408.en_US
dc.description.abstractObstructions of the ureter lumen can originate from intrinsic or extrinsic factors, such as kidney stones, tumours, or strictures. These can affect the physiological flow of urine from the kidneys to the bladder, potentially causing infection, pain, and kidney failure. To overcome these complications, ureteral stents are often deployed clinically in order to temporarily re-establish urinary flow. Despite their clinical benefits, stents are prone to encrustation and biofilm formation that lead to reduced quality of life for patients; however, the mechanisms underlying the formation of crystalline biofilms in stents are not yet fully understood. In this study, we developed microfluidic-based devices replicating the urodynamic field within different configurations of an occluded and stented ureter. We employed computational fluid dynamic simulations to characterise the flow dynamic field within these models and investigated bacterial attachment (Pseudomonas fluorescens) by means of crystal violet staining and fluorescence microscopy. We identified the presence of hydrodynamic cavities in the vicinity of a ureteric occlusion, which were characterised by low levels of wall shear stress (WSS < 40 mPa), and observed that initiation of bacterial attachment occurred in these specific regions of the stented ureter. Notably, the bacterial coverage area was directly proportional to the number of cavities present in the model. Fluorescence microscopy confirmed that the number density of bacteria was greater within cavities (3 bacteria·mm-2) when compared to side-holes of the stent (1 bacterium·mm-2) or its luminal surface (0.12·bacteria mm-2). These findings informed the design of a novel technological solution against bacterial attachment, which reduces the extent of cavity flow and increases wall shear stress over the stent's surface.en_US
dc.rights© This published version is made available under the CC-BY 4.0 license.en_US
dc.titleA Microfluidic-Based Investigation of Bacterial Attachment in Ureteral Stentsen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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