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dc.contributor.authorAllkja, Jontana
dc.contributor.authorvan Charante, Frits
dc.contributor.authorAizawa, Juliana
dc.contributor.authorReigada, Ines
dc.contributor.authorGuarch-Perez, Clara
dc.contributor.authorVazquez-Rodriguez, Jesus Augusto
dc.contributor.authorCos, Paul
dc.contributor.authorCoenye, Tom
dc.contributor.authorFallarero, Adyary
dc.contributor.authorZaat, Sebastian A. J.
dc.contributor.authorFelici, Antonio
dc.contributor.authorFerrari, Livia
dc.contributor.authorAzevado, Nuno F.
dc.contributor.authorParker, Albert E.
dc.contributor.authorGoeres, Darla M.
dc.identifier.citationAllkja, Jontana, Frits van Charante, Juliana Aizawa, Inés Reigada, Clara Guarch-Pérez, Jesus Augusto Vazquez-Rodriguez, Paul Cos, et al. “Interlaboratory Study for the Evaluation of Three Microtiter Plate-Based Biofilm Quantification Methods.” Scientific Reports 11, no. 1 (July 2, 2021). doi:10.1038/s41598-021-93115-w.en_US
dc.description.abstractMicrotiter plate methods are commonly used for biofilm assessment. However, results obtained with these methods have often been difficult to reproduce. Hence, it is important to obtain a better understanding of the repeatability and reproducibility of these methods. An interlaboratory study was performed in five different laboratories to evaluate the reproducibility and responsiveness of three methods to quantify Staphylococcus aureus biofilm formation in 96-well microtiter plates: crystal violet, resazurin, and plate counts. An inter-lab protocol was developed for the study. The protocol was separated into three steps: biofilm growth, biofilm challenge, biofilm assessment. For control experiments participants performed the growth and assessment steps only. For treatment experiments, all three steps were performed and the efficacy of sodium hypochlorite (NaOCl) in killing S. aureus biofilms was evaluated. In control experiments, on the log10-scale, the reproducibility SD (SR) was 0.44 for crystal violet, 0.53 for resazurin, and 0.92 for the plate counts. In the treatment experiments, plate counts had the best responsiveness to different levels of efficacy and also the best reproducibility with respect to responsiveness (Slope/SR = 1.02), making it the more reliable method to use in an antimicrobial efficacy test. This study showed that the microtiter plate is a versatile and easy-to-use biofilm reactor, which exhibits good repeatability and reproducibility for different types of assessment methods, as long as a suitable experimental design and statistical analysis is applied.en_US
dc.description.sponsorshipResearch funded by Horizon 2020 (722467) | Fundacao para a Ciencia e a Tecnologia (UIDB/00511/2020)en_US
dc.rights© This final published version is made available under the CC-BY 4.0 license.en_US
dc.titleInterlaboratory study for the evaluation of three microtiter plate-based biofilm quantification methodsen_US
mus.citation.journaltitleScientific Reportsen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentMathematical Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US

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