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dc.contributor.authorUrbaniak, Camilla
dc.contributor.authorWong, Season
dc.contributor.authorTighe, Scott
dc.contributor.authorArumugam, Arunkumar
dc.contributor.authorLiu, Bo
dc.contributor.authorParker, Ceth W.
dc.contributor.authorWood, Jason M.
dc.contributor.authorSingh, Nitin K.
dc.contributor.authorSkorupa, Dana J.
dc.contributor.authorPeyton, Brent M.
dc.contributor.authorJenson, Ryan
dc.contributor.authorKarouia, Fathi
dc.contributor.authorDragon, Julie
dc.contributor.authorVenkateswaran, Kasthuri
dc.date.accessioned2021-10-05T17:29:49Z
dc.date.available2021-10-05T17:29:49Z
dc.date.issued2020-08
dc.identifier.citationUrbaniak, Camilla, Season Wong, Scott Tighe, Arunkumar Arumugam, Bo Liu, Ceth W. Parker, Jason M. Wood, et al. “Validating an Automated Nucleic Acid Extraction Device for Omics in Space Using Whole Cell Microbial Reference Standards.” Frontiers in Microbiology 11 (August 21, 2020). doi:10.3389/fmicb.2020.01909.en_US
dc.identifier.issn1664-302X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/16466
dc.description.abstractNASA has made great strides in the past five years to develop a suite of instruments for the International Space Station in order to perform molecular biology in space. However, a key piece of equipment that has been lacking is an instrument that can extract nucleic acids from an array of complex human and environmental samples. The Omics in Space team has developed the μTitan (simulated micro(μ) gravity tested instrument for automated nucleic acid) system capable of automated, streamlined, nucleic acid extraction that is adapted for use under microgravity. The μTitan system was validated using a whole cell microbial reference (WCMR) standard comprised of a suspension of nine bacterial strains, titrated to concentrations that would challenge the performance of the instrument, as well as to determine the detection limits for isolating DNA. Quantitative assessment of system performance was measured by comparing instrument input challenge dose vs recovery by Qubit spectrofluorometry, qPCR, Bioanalyzer, and Next Generation Sequencing. Overall, results indicate that the μTitan system performs equal to or greater than a similar commercially available, earth-based, automated nucleic acid extraction device. The μTitan system was also tested in Yellowstone National Park (YNP) with the WCMR, to mimic a remote setting, with limited resources. The performance of the device at YNP was comparable to that in a laboratory setting. Such a portable, field-deployable, nucleic extraction system will be valuable for environmental microbiology, as well as in health care diagnostics.en_US
dc.language.isoen_USen_US
dc.rights© This final published version is made available under the CC-BY 4.0 license.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.titleValidating an Automated Nucleic Acid Extraction Device for Omics in Space Using Whole Cell Microbial Reference Standardsen_US
dc.typeArticleen_US
mus.citation.journaltitleFrontiers in Microbiologyen_US
mus.citation.volume11en_US
mus.identifier.doi10.3389/fmicb.2020.01909en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.researchgroupThermal Biology Institute (TBI).en_US
mus.data.thumbpage4en_US


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