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dc.contributor.authorZambare, Neerja M.
dc.contributor.authorNaser, Nada Y.
dc.contributor.authorGerlach, Robin
dc.contributor.authorChang, Connie B.
dc.date.accessioned2021-12-03T22:15:08Z
dc.date.available2021-12-03T22:15:08Z
dc.date.issued2020-10
dc.identifier.citationZambare, N.M., Naser, N.Y., Gerlach, R. et al. Mineralogy of microbially induced calcium carbonate precipitates formed using single cell drop-based microfluidics. Scientific Reports 10, 17535 (2020). doi: 10.1038/s41598-020-73870-yen_US
dc.identifier.issn2045-2322
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/16560
dc.description.abstractMicrobe-mineral interactions are ubiquitous and can facilitate major biogeochemical reactions that drive dynamic Earth processes such as rock formation. One example is microbially induced calcium carbonate precipitation (MICP) in which microbial activity leads to the formation of calcium carbonate precipitates. A majority of MICP studies have been conducted at the mesoscale but fundamental questions persist regarding the mechanisms of cell encapsulation and mineral polymorphism. Here, we are the first to investigate and characterize precipitates on the microscale formed by MICP starting from single ureolytic E. coli MJK2 cells in 25 µm diameter drops. Mineral precipitation was observed over time and cells surrounded by calcium carbonate precipitates were observed under hydrated conditions. Using Raman microspectroscopy, amorphous calcium carbonate (ACC) was observed first in the drops, followed by vaterite formation. ACC and vaterite remained stable for up to 4 days, possibly due to the presence of organics. The vaterite precipitates exhibited a dense interior structure with a grainy exterior when examined using electron microscopy. Autofluorescence of these precipitates was observed possibly indicating the development of a calcite phase. The developed approach provides an avenue for future investigations surrounding fundamental processes such as precipitate nucleation on bacteria, microbe-mineral interactions, and polymorph transitions.en_US
dc.language.isoen_USen_US
dc.rights© This final published version is made available under the CC-BY 4.0 license.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleMineralogy of microbially induced calcium carbonate precipitates formed using single cell drop-based microfluidicsen_US
dc.typeArticleen_US
mus.citation.journaltitleScientific Reportsen_US
mus.citation.volume10en_US
mus.identifier.doi10.1038/s41598-020-73870-yen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage5en_US


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