Show simple item record

dc.contributor.authorPark, Heejoon
dc.contributor.authorPatel, Ayushi
dc.contributor.authorHunt, Kristopher A.
dc.contributor.authorHenson, Michael A.
dc.contributor.authorCarlson, Ross P.
dc.date.accessioned2021-12-03T22:48:36Z
dc.date.available2021-12-03T22:48:36Z
dc.date.issued2020-12
dc.identifier.citationPark, H., Patel, A., Hunt, K.A. et al. Artificial consortium demonstrates emergent properties of enhanced cellulosic-sugar degradation and biofuel synthesis. npj Biofilms Microbiomes 6, 59 (2020). doi: 10.1038/s41522-020-00170-8en_US
dc.identifier.issn2055-5008
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/16562
dc.description.abstractPlanktonic cultures, of a rationally designed consortium, demonstrated emergent properties that exceeded the sums of monoculture properties, including a >200% increase in cellobiose catabolism, a >100% increase in glycerol catabolism, a >800% increase in ethanol production, and a >120% increase in biomass productivity. The consortium was designed to have a primary and secondary-resource specialist that used crossfeeding with a positive feedback mechanism, division of labor, and nutrient and energy transfer via necromass catabolism. The primary resource specialist was Clostridium phytofermentans (a.k.a. Lachnoclostridium phytofermentans), a cellulolytic, obligate anaerobe. The secondary-resource specialist was Escherichia coli, a versatile, facultative anaerobe, which can ferment glycerol and byproducts of cellobiose catabolism. The consortium also demonstrated emergent properties of enhanced biomass accumulation when grown as biofilms, which created high cell density communities with gradients of species along the vertical axis. Consortium biofilms were robust to oxic perturbations with E. coli consuming O2, creating an anoxic environment for C. phytofermentans. Anoxic/oxic cycling further enhanced biomass productivity of the biofilm consortium, increasing biomass accumulation ~250% over the sum of the monoculture biofilms. Consortium emergent properties were credited to several synergistic mechanisms. E. coli consumed inhibitory byproducts from cellobiose catabolism, driving higher C. phytofermentans growth and higher cellulolytic enzyme production, which in turn provided more substrate for E. coli. E. coli necromass enhanced C. phytofermentans growth while C. phytofermentans necromass aided E. coli growth via the release of peptides and amino acids, respectively. In aggregate, temporal cycling of necromass constituents increased flux of cellulose-derived resources through the consortium. The study establishes a consortia-based, bioprocessing strategy built on naturally occurring interactions for improved conversion of cellulose-derived sugars into bioproducts.en_US
dc.language.isoen_USen_US
dc.rights© This final published version is made available under the CC-BY 4.0 license.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleArtificial consortium demonstrates emergent properties of enhanced cellulosic-sugar degradation and biofuel synthesisen_US
dc.typeArticleen_US
mus.citation.journaltitlenpj Biofilms Microbiomesen_US
mus.citation.volume6en_US
mus.identifier.doi10.1038/s41522-020-00170-8en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage3en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

© This final published version is made available under the CC-BY 4.0 license.
Except where otherwise noted, this item's license is described as © This final published version is made available under the CC-BY 4.0 license.

MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.