Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates
Loading...
Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier BV
Abstract
The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.
Description
Keywords
Citation
Jontana Allkja, Thomas Bjarnsholt, Tom Coenye, Paul Cos, Adyary Fallarero, Joe J. Harrison, Susana P. Lopes, Antonio Oliver, Maria Olivia Pereira, Gordon Ramage, Mark E. Shirtliff, Paul Stoodley, Jeremy S. Webb, Sebastian A.J. Zaat, Darla M. Goeres, Nuno Filipe Azevedo (2020). Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates. Biofilm, 2, 100010. https://doi.org/10.1016/j.bioflm.2019.100010