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dc.contributor.authorAlhede, Maria
dc.contributor.authorAlhede, Morten
dc.contributor.authorQvortrup, Klaus
dc.contributor.authorKragh, Kasper Nørskov
dc.contributor.authorJensen, Peter Østrup
dc.contributor.authorStewart, Philip Shook
dc.contributor.authorBjarnsholt, Thomas
dc.date.accessioned2022-05-17T20:43:58Z
dc.date.available2022-05-17T20:43:58Z
dc.date.issued2020-03
dc.identifier.citationAlhede, M., Alhede, M., Qvortrup, K., Kragh, K. N., Jensen, P. Ø., Stewart, P. S., & Bjarnsholt, T. (2020). The origin of extracellular DNA in bacterial biofilm infections in vivo. Pathogens and disease, 78(2), ftaa018.en_US
dc.identifier.issn2049-632X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/16792
dc.description.abstractExtracellular DNA (eDNA) plays an important role in both the aggregation of bacteria and in the interaction of the resulting biofilms with polymorphonuclear leukocytes (PMNs) during an inflammatory response. Here, transmission electron and confocal scanning laser microscopy were used to examine the interaction between biofilms of Pseudomonas aeruginosa and PMNs in a murine implant model and in lung tissue from chronically infected cystic fibrosis patients. PNA FISH, DNA staining, labeling of PMN DNA with a thymidine analogue and immunohistochemistry were applied to localize bacteria, eDNA, PMN-derived eDNA, PMN-derived histone H3 (H3), neutrophil elastase (NE) and citrullinated H3 (citH3). Host-derived eDNA was observed surrounding bacterial biofilms but not within the biofilms. H3 localized to the lining of biofilms while NE was found throughout biofilms. CitH3, a marker for neutrophil extracellular traps (NETs) was detected only sporadically indicating that most host-derived eDNA in vivo was not a result of NETosis. Together these observations show that, in these in vivo biofilm infections with P. aeruginosa, the majority of eDNA is found external to the biofilm and derives from the host.en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.rightsThe Author(s) 2019. Published by Oxford University Press on behalf of FEMS. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en_US
dc.titleThe origin of extracellular DNA in bacterial biofilm infections in vivoen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage15en_US
mus.citation.issue2en_US
mus.citation.journaltitlePathogens and Diseaseen_US
mus.citation.volume78en_US
mus.identifier.doi10.1093/femspd/ftaa018en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage3en_US


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The Author(s) 2019. Published by Oxford University Press on behalf of FEMS. This is an Open Access article distributed under the terms of the
Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use,
distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as The Author(s) 2019. Published by Oxford University Press on behalf of FEMS. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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