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dc.contributor.authorAltae-Tran, Han
dc.contributor.authorKannan, Soumya
dc.contributor.authorDemircioglu, F. Esra
dc.contributor.authorOshiro, Rachel
dc.contributor.authorNety, Suchita P.
dc.contributor.authorMcKay, Luke J.
dc.contributor.authorDlakić, Mensur
dc.contributor.authorInskeep, William P.
dc.contributor.authorMakarova, Kira S.
dc.contributor.authorMacrae, Rhiannon K.
dc.contributor.authorKoonin, Eugene V.
dc.contributor.authorZhang, Feng
dc.identifier.citationAltae-Tran, H., Kannan, S., Demircioglu, F. E., Oshiro, R., Nety, S. P., McKay, L. J., ... & Zhang, F. (2021). The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Science, 374(6563), 57-65.en_US
dc.description.abstractIscB proteins are putative nucleases encoded in a distinct family of IS200/IS605 transposons and are likely ancestors of the RNA-guided endonuclease Cas9, but the functions of IscB and its interactions with any RNA remain uncharacterized. Using evolutionary analysis, RNA sequencing, and biochemical experiments, we reconstructed the evolution of CRISPR-Cas9 systems from IS200/IS605 transposons. We found that IscB uses a single noncoding RNA for RNA-guided cleavage of double-stranded DNA and can be harnessed for genome editing in human cells. We also demonstrate the RNA-guided nuclease activity of TnpB, another IS200/IS605 transposon-encoded protein and the likely ancestor of Cas12 endonucleases. This work reveals a widespread class of transposon-encoded RNA-guided nucleases, which we name OMEGA (obligate mobile element–guided activity), with strong potential for developing as biotechnologies.en_US
dc.publisherAmerican Association for the Advancement of Scienceen_US
dc.titleThe widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleasesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.departmentMicrobiology & Cell Biology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.researchgroupThermal Biology Institute (TBI).en_US

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