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dc.contributor.authorSchweitzer, Hannah D.
dc.contributor.authorSmith, Heidi J.
dc.contributor.authorBarnhart, Elliott P.
dc.contributor.authorMcKay, Luke J.
dc.contributor.authorGerlach, Robin
dc.contributor.authorCunningham, Alfred B.
dc.contributor.authorMalmstrom, Rex R.
dc.contributor.authorGoudeau, Danielle
dc.contributor.authorFields, Matthew W.
dc.identifier.citationSchweitzer, H. D., Smith, H. J., Barnhart, E. P., McKay, L. J., Gerlach, R., Cunningham, A. B., ... & Fields, M. W. (2022). Subsurface hydrocarbon degradation strategies in low-and high-sulfate coal seam communities identified with activity-based metagenomics. NPJ biofilms and microbiomes, 8(1), 1-10.en_US
dc.description.abstractEnvironmentally relevant metagenomes and BONCAT-FACS derived translationally active metagenomes from Powder River Basin coal seams were investigated to elucidate potential genes and functional groups involved in hydrocarbon degradation to methane in coal seams with high- and low-sulfate levels. An advanced subsurface environmental sampler allowed the establishment of coal-associated microbial communities under in situ conditions for metagenomic analyses from environmental and translationally active populations. Metagenomic sequencing demonstrated that biosurfactants, aerobic dioxygenases, and anaerobic phenol degradation pathways were present in active populations across the sampled coal seams. In particular, results suggested the importance of anaerobic degradation pathways under high-sulfate conditions with an emphasis on fumarate addition. Under low-sulfate conditions, a mixture of both aerobic and anaerobic pathways was observed but with a predominance of aerobic dioxygenases. The putative low-molecular-weight biosurfactant, lichysein, appeared to play a more important role compared to rhamnolipids. The methods used in this study—subsurface environmental samplers in combination with metagenomic sequencing of both total and translationally active metagenomes—offer a deeper and environmentally relevant perspective on community genetic potential from coal seams poised at different redox conditions broadening the understanding of degradation strategies for subsurface carbon.en_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.titleSubsurface hydrocarbon degradation strategies in low- and high-sulfate coal seam communities identified with activity-based metagenomicsen_US
mus.citation.journaltitleNpj Biofilms and Microbiomesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentCivil Engineering.en_US
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.departmentMicrobiology & Cell Biology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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