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dc.contributor.authorThomas, Merrilee
dc.contributor.authorHughes, Thomas E.
dc.identifier.citationThomas M, Hughes TE (2020) Optically activated, customizable, excitable cells. PLoS ONE 15(12): e0229051.en_US
dc.description.abstractGenetically encoded fluorescent biosensors are powerful tools for studying complex signaling in the nervous system, and now both Ca2+ and voltage sensors are available to study the signaling behavior of entire neural circuits. There is a pressing need for improved sensors, but improving them is challenging because testing them involves a low throughput, labor-intensive processes. Our goal was to create synthetic, excitable cells that can be activated with brief pulses of blue light and serve as a medium throughput platform for screening the next generation of sensors. In this live cell system, blue light activates an adenylyl cyclase enzyme (bPAC) that increases intracellular cAMP (Stierl M et al. 2011). In turn, the cAMP opens a cAMP-gated ion channel. This produces slow, whole-cell Ca2+ transients and voltage changes. To increase the speed of these transients, we add the inwardly rectifying potassium channel Kir2.1, the bacterial voltage-gated sodium channel NAVROSD, and Connexin-43. The result is a highly reproducible, medium-throughput, live cell system that can be used to screen voltage and Ca2+ sensors.en_US
dc.publisherPublic Library of Scienceen_US
dc.titleOptically activated, customizable, excitable cellsen_US
mus.citation.journaltitlePLoS ONEen_US
mus.identifier.doi10.1371/journal. pone.0229051en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentMicrobiology & Cell Biology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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