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dc.contributor.authorFuchs, Amanda
dc.contributor.authorMiller, Isaac
dc.contributor.authorSchiller, Sage
dc.contributor.authorAmmons, Mary
dc.contributor.authorEilers, Brian
dc.contributor.authorTripet, Brian
dc.contributor.authorCopie, Valerie
dc.identifier.citationFuchs, A. L., Miller, I. R., Schiller, S. M., Ammons, M. C. B., Eilers, B., Tripet, B., & Copié, V. (2020). Pseudomonas aeruginosa planktonic-and biofilm-conditioned media elicit discrete metabolic responses in human macrophages. Cells, 9(10), 2260.en_US
dc.description.abstractMacrophages (MΦs) are prevalent innate immune cells, present throughout human bodily tissues where they orchestrate innate and adaptive immune responses to maintain cellular homeostasis. MΦs have the capacity to display a wide array of functional phenotypes due to different microenvironmental cues, particularly soluble bacterial secretory products. Recent evidence has emerged demonstrating that metabolism supports MΦ function and plasticity, in addition to energy and biomolecular precursor production. In this study, 1D 1H-NMR-based metabolomics was used to identify the metabolic pathways that are differentially altered following primary human monocyte-derived MΦ exposure to P. aeruginosa planktonic- and biofilm-conditioned media (PCM and BCM). Metabolic profiling of PCM- and BCM-exposed MΦs indicated a significant increase in glycolytic metabolism, purine biosynthesis, and inositol phosphate metabolism. In addition, these metabolic patterns suggested that BCM-exposed MΦs exhibit a hyperinflammatory metabolic profile with reduced glycerol metabolism and elevated catabolism of lactate and amino acids, relative to PCM-exposed MΦs. Altogether, our study reveals novel findings concerning the metabolic modulation of human MΦs after exposure to secretory microbial products and contributes additional knowledge to the field of immunometabolism in MΦs.en_US
dc.publisherMDPI AGen_US
dc.titlePseudomonas aeruginosa Planktonic- and Biofilm-Conditioned Media Elicit Discrete Metabolic Responses in Human Macrophagesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentMicrobiology & Cell Biology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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