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dc.contributor.authorMessmer, Mitch W.
dc.contributor.authorDieser, Markus
dc.contributor.authorSmith, Heidi J.
dc.contributor.authorParker, Albert E.
dc.contributor.authorForeman, Christine M.
dc.identifier.citationMessmer, M. W., Dieser, M., Smith, H. J., Parker, A. E., & Foreman, C. M. (2022). Investigation of Raman spectroscopic signatures with multivariate statistics: an approach for cataloguing microbial biosignatures. Astrobiology, 22(1), 14-24.en_US
dc.descriptionFinal publication is available from Mary Ann Liebert, Inc., publishers
dc.description.abstractSpectroscopic instruments are increasingly being implemented in the search for extraterrestrial life. However, microstructural spectral analyses of alien environments could prove difficult without knowledge on the molecular identification of individual spectral signatures. To bridge this gap, we introduce unsupervised K-means clustering as a statistical approach to discern spectral patterns of biosignatures without prior knowledge of spectral regions of biomolecules. Spectral profiles of bacterial isolates from analogous polar ice sheets were measured with Raman spectroscopy. Raman analysis identified carotenoid and violacein pigments, and key cellular features including saturated and unsaturated fats, triacylglycerols, and proteins. Principal component analysis and targeted spectra integration biplot analysis revealed that the clustering of bacterial isolates was attributed to spectral biosignatures influenced by carotenoid pigments and ratio of unsaturated/saturated fat peaks. Unsupervised K-means clustering highlighted the prevalence of the corresponding spectral peaks, while subsequent supervised permutational multivariate analysis of variance provided statistical validation for spectral differences associated with the identified cellular features. Establishing a validated catalog of spectral signatures of analogous biotic and abiotic materials, in combination with targeted supervised tools, could prove effective at identifying extant biosignatures.en_US
dc.publisherMary Ann Liebert Incen_US
dc.rightscopyright Mary Ann Liebert Inc 2021en_US
dc.subjectraman spectroscopyen_US
dc.subjectenvironmental isolatesen_US
dc.subjectpolar microbesen_US
dc.subjectexraterrestrial lifeen_US
dc.subjectunsupervised k-means clusteringen_US
dc.titleInvestigation of Raman Spectroscopic Signatures with Multivariate Statistics: An Approach for Cataloguing Microbial Biosignaturesen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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