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dc.contributor.authorAttri, Komal
dc.contributor.authorZhang, Zijie
dc.contributor.authorSingh, Atinder
dc.contributor.authorSharrock, Robert A.
dc.contributor.authorXie, Zhixin
dc.identifier.citationAttri, K., Zhang, Z., Singh, A., Sharrock, R. A., & Xie, Z. (2022). Rapid sequence and functional diversification of a miRNA superfamily targeting calcium signaling components in seed plants. New Phytologist.en_US
dc.descriptionThis is the peer reviewed version of the following article: [Rapid sequence and functional diversification of a miRNA superfamily targeting calcium signaling components in seed plants. New Phytologist 235, 3 p1082-1095 (2022)], which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions:
dc.description.abstractMicroRNA (miRNA)-directed posttranscriptional gene silencing (miR-PTGS) is an integral component of gene regulatory networks governing plant development and responses to the environment. The sequence homology between Sly-miR4376, a miRNA common to Solanaceae and reported to target autoinhibited Ca2+-ATPase 10 (ACA10) messenger RNA (mRNA) in tomato, and Arabidopsis miR391 (Ath-miR391), previously annotated as a nonconserved member of the deeply conserved miR390 family, has prompted us to revisit the function of Ath-miR391, as well as its regulatory conservation. A combination of genetic, molecular, and bioinformatic analyses revealed a hidden conservation for miR-PTGS of ACA10 homologs in spermatophytes. We found that the Arabidopsis ACA10 mRNA undergoes miR391-directed cleavage in vivo. Furthermore, transgenic overexpression of miR391 recapitulated the compact inflorescence (cif) phenotypes characteristic of ACA10 loss-of function mutants, due to miR391-directed PTGS of ACA10. Significantly, comprehensive data mining revealed robust evidence for widespread PTGS of ACA10 homologs directed by a superfamily of related miRNAs sharing a conserved sequence core. Intriguingly, the ACA-targeting miRNAs in Poaceae also direct PTGS for calmodulin-like proteins which are putative Ca2+ sensors. The PTGS of ACA10 homologs is therefore directed by a miRNA superfamily that is of ancient origin and has undergone rapid sequence diversification associated with functional innovation.en_US
dc.rightscopyright Wiley 2022en_US
dc.subjectautoinhibitedCa2+-ATPase (ACA)en_US
dc.subjectcalcium signalingen_US
dc.subjectcalmodulin-like (CML),en_US
dc.subjectEF hand (EF-h)en_US
dc.subjectposttranscriptional gene silencing(PTGS)en_US
dc.titleRapid sequence and functional diversification of a miRNA superfamily targeting calcium signaling components in seed plantsen_US
mus.citation.journaltitleNew Phytologisten_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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