Show simple item record

dc.contributor.authorHahn, Alyssa K.
dc.contributor.authorRawle, Rachel A.
dc.contributor.authorBothner, Brian
dc.contributor.authorPrado Lopes, Erika Barboza
dc.contributor.authorGriffin, Timothy M.
dc.contributor.authorJune, Ronald K.
dc.identifier.citationHahn, A. K., Rawle, R. A., Bothner, B., Lopes, E. B. P., Griffin, T. M., & June, R. K. (2022). In vivo mechanotransduction: Effect of acute exercise on the metabolomic profiles of mouse synovial fluid. Osteoarthritis and Cartilage Open, 4(1), 100228.en_US
dc.description.abstractObjective. Exercise is known to induce beneficial effects in synovial joints. However, the mechanisms underlying these are unclear. Synovial joints experience repeated mechanical loading during exercise. These mechanical stimuli are transduced into biological responses through cellular mechanotransduction. Mechanotransduction in synovial joints is typically studied in tissues. However, synovial fluid directly contacts all components of the joint, and thus may produce a whole-joint picture of the mechanotransduction response to loading. The objective of this study was to determine if metabolic phenotypes are present in the synovial fluid after acute exercise as a first step to understanding the beneficial effects of exercise on the joint. Material and methods. Mice underwent a single night of voluntary wheel running or standard housing and synovial fluid was harvested for global metabolomic profiling by LC-MS. Hierarchical unsupervised clustering, partial least squares discriminant, and pathway analysis provided insight into exercise-induced mechanotransduction. Results. Acute exercise produced a distinct metabolic phenotype in synovial fluid. Mechanosensitive metabolites included coenzyme A derivatives, prostaglandin derivatives, phospholipid species, tryptophan, methionine, vitamin D3, fatty acids, and thiocholesterol. Enrichment analysis identified several pathways previously linked to exercise including amino acid metabolism, inflammatory pathways, citrulline-nitric oxide cycle, catecholamine biosynthesis, ubiquinol biosynthesis, and phospholipid metabolism. Conclusion. To our knowledge, this is the first study to investigate metabolomic profiles of synovial fluid during in vivo mechanotransduction. These profiles indicate that exercise induced stress-response processes including both pro- and anti-inflammatory pathways. Further research will expand these results and define the relationship between the synovial fluid and the serum.en_US
dc.publisherElsevier BVen_US
dc.subjectvivo mechanotransductionen_US
dc.subjectacute exerciseen_US
dc.subjectmetabolomic profilesen_US
dc.subjectmouse synovial fluiden_US
dc.titleIn vivo mechanotransduction: Effect of acute exercise on the metabolomic profiles of mouse synovial fluiden_US
mus.citation.journaltitleOsteoarthritis and Cartilage Openen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.universityMontana State University - Bozemanen_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

Except where otherwise noted, this item's license is described as cc-by

MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.