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dc.contributor.authorYang, Qingqing
dc.contributor.authorYan, Qing
dc.contributor.authorZhang, Bo
dc.contributor.authorZhang, Li-qun
dc.contributor.authorWu, Xiaogang
dc.date.accessioned2023-11-09T22:48:55Z
dc.date.available2023-11-09T22:48:55Z
dc.date.issued2023-07
dc.identifier.citationYang, Q., Yan, Q., Zhang, B., Zhang, L. Q., & Wu, X. (2023). Citrate Synthase GltA Modulates the 2, 4-Diacetylphloroglucinol Biosynthesis of Pseudomonas fluorescens 2P24 and is Essential for the Biocontrol Capacity. Journal of Agricultural and Food Chemistry, 71(31), 11892-11901.en_US
dc.identifier.issn1520-5118
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/18193
dc.description.abstractCarbon metabolism is critical for microbial physiology and remarkably affects the outcome of secondary metabolite production. The production of 2,4-diacetylphloroglucinol (2,4-DAPG), a bacterial secondary metabolite with a broad spectrum of antibiotic activity, is a major mechanism used by the soil bacterium Pseudomonas fluorescens 2P24 to inhibit the growth of plant pathogens and control disease occurrence. Strain 2P24 has evolved a complex signaling cascade to regulate the production of 2,4-DAPG. However, the role of the central carbon metabolism in modulating 2,4-DAPG production has not been fully determined. In this study, we report that the gltA gene, which encodes citrate synthase, affects the expression of the 2,4-DAPG biosynthesis gene and is essential for the biocontrol capacity of strain 2P24. Our data showed that the mutation of gltA remarkably decreased the biosynthesis of 2,4-DAPG. Consistent with this result, the addition of citrate in strain 2P24 resulted in increased 2,4-DAPG production and decreased levels of RsmA and RsmE. In comparison with the wild-type strain, the gltA mutant was severely impaired in terms of biocontrol activity against the bacterial wilt disease of tomato plants caused by Ralstonia solanacearum. Moreover, the gltA mutant exhibited increased antioxidant activity, and the expression of oxidative, stress-associated genes, including ahpB, katB, and oxyR, was significantly upregulated in the gltA mutant compared to the wild-type strain. Overall, our data indicate that the citrate synthase GltA plays an important role in the production of 2,4-DAPG and oxidative stress and is required for biocontrol capacity.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightscc-by-nc-nden_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectPseudomonas fluorescensen_US
dc.subject2,4-DAPGen_US
dc.subjectgltAen_US
dc.subjectRsmA/RsmEen_US
dc.subjectbiocontrolen_US
dc.titleCitrate Synthase GltA Modulates the 2,4-Diacetylphloroglucinol Biosynthesis of Pseudomonas fluorescens 2P24 and is Essential for the Biocontrol Capacityen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage10en_US
mus.citation.issue31en_US
mus.citation.journaltitleJournal of Agricultural and Food Chemistryen_US
mus.citation.volume71en_US
mus.identifier.doi10.1021/acs.jafc.3c03051en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage5en_US


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