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dc.contributor.advisorChairperson, Graduate Committee: Mark Young.en
dc.contributor.authorPomwised, Rattanaruji.en
dc.date.accessioned2013-06-25T18:37:28Z
dc.date.available2013-06-25T18:37:28Z
dc.date.issued2007en
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/2066
dc.description.abstractCapsular polysaccharide (CPS) of Streptococcus group B (GBS) is a poor immunogen and functions as T cell independent antigen, eliciting low IgG antibody with deficient immunologic memory. We previously identified a peptide, S9, which mimics CPS of type III GBS. Here we have taken steps to develop the mimetic peptide as a vaccine against GBS group III. We enhanced the immunogenicity of the peptide by presenting it on the coat protein of Cowpea Chlorotic Mottle Virus (CCMV). And we searched for better mimetic peptides by constructing a secondary phage display library. To accomplish the first goal, DNA encoding S9 was cloned into five constructions CCMV coat protein loops using recombinant DNA techniques. The results indicated that inserting the S9 peptide sequence into CCMV coat protein loops disrupted virus and virus-like particle assembly. Therefore the S9 peptide was conjugated to CCMV coat protein using chemical linkers. The CCMV-S9 conjugation products remained intact as monomer virions.en
dc.language.isoengen
dc.publisherMontana State University - Bozeman, College of Letters & Scienceen
dc.subject.lcshMimicry (Chemistry)en
dc.subject.lcshBacteriophages.en
dc.subject.lcshStreptococcus.en
dc.subject.lcshPeptides.en
dc.titleStudies of peptide mimicry of the group B Streptococcus type III capsular polysaccharide antigen
dc.typeDissertation
dc.rights.holderCopyright Rattanaruji Pomwised 2007en
thesis.catalog.ckey1290709en
thesis.degree.committeemembersMembers, Graduate Committee: Seth Pincus (co-chair); Martin Teintze; James Burritt; Jean Starkey; Richard Smithen
thesis.degree.departmentMicrobiology & Immunology.en
thesis.degree.genreDissertationen
thesis.degree.namePhDen
thesis.format.extentfirstpage1en
thesis.format.extentlastpage239en
mus.identifier.categoryLife Sciences & Earth Sciences
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US


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