Montana State University (MSU) Library in Bozeman Montana State University - Home Montana State University Libraries - Home
    • Login
    View Item 
    •   ScholarWorks Home
    • Scholarship & Research
    • Theses and Dissertations at Montana State University (MSU)
    • Theses and Dissertations at Montana State University (MSU)
    • View Item
    •   ScholarWorks Home
    • Scholarship & Research
    • Theses and Dissertations at Montana State University (MSU)
    • Theses and Dissertations at Montana State University (MSU)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Mannose/tempo functionalized pamam dendrimers : their relative locations and components of affinity towards Concanavalin A

    Thumbnail
    View/Open
    SamuelsonL0504.pdf (4.540Mb)
    Date
    2004
    Author
    Samuelson, Lynn Elizabeth.
    Metadata
    Show full item record
    Abstract
    Surface functionalized dendrimers are being used for several applications including the study of protein-carbohydrate interactions. Mannose-functionalized dendrimers with varying concentrations of saccharides on the dendrimer surface were synthesized. Spin labels (2,2,6,6-tetramethylpiperidine N-oxide) were incorporated onto the dendrimer's surface as well. Linebroadening effects in the EPR spectra of these compounds allowed us to determine the distance between spin labels (and thus between carbohydrates). The mannose-spin labeled functionalized dendrimers were further studied to determine effects of the spin label in hemagglutination inhibition assays. Affinity chromatography was employed to separate any mixture of compounds based on their affinity towards Concanavalin A, a mannose specific protein. The spin label on these compounds was used to study the relative conformations of the different compounds obtained from the affinity column. Synthesis of glucosamine funtionalized dendrimers was undertaken unsuccessfully. Had the synthesis been a success, TEMPO residues would have been attached to the amino sugar. EPR studies would have been used to determine the relative locations of the TEMPO labeled carbohydrates directly.
    URI
    https://scholarworks.montana.edu/xmlui/handle/1/2173
    Collections
    • Theses and Dissertations at Montana State University (MSU)

    MSU uses DSpace software, copyright © 2002-2013  Duraspace
    Contact Us | Send Feedback
     

     

    Browse

    All of ScholarWorksCommunities & CollectionsBy Issue DateAuthorsTitlesDepartmentsItem TypeThis CollectionBy Issue DateAuthorsTitlesDepartmentsItem Type

    My Account

    Login

    Guidelines & Policies

    AllFor authorsWhy to submitHow to submit

    Statistics

    View Usage Statistics

    MSU uses DSpace software, copyright © 2002-2013  Duraspace
    Contact Us | Send Feedback