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dc.contributor.advisorChairperson, Graduate Committee: Brent M. Peyton; Robin Gerlach (co-chair)en
dc.contributor.authorVanEngelen, Michael Roberten
dc.description.abstractUranium (U), as the uranyl ion (UO 2 ²+), is a widely distributed contaminant at several Department of Energy (DOE) sites, former war zones, and across the globe. Although many U remediation efforts depend on U-bacterial interactions, little information regarding U-bacterial interactions resolved at the molecular level exist. In this study, experiments were performed aimed at understanding the effect of molecular UO 2 ²+ speciation on bacterial bioaccumulation and toxicity using an environmental Pseudomonas sp. isolate. Results showed that the charge and stability of UO 2 ²+ species largely controlled the extent of UO 2 ²+ bioaccumulation and UO 2 ²+ toxicity, respectively. Further experimentation, including a combination of in vivo, in vitro, and in silico studies, revealed a specific mechanism of UO 2 ²+ toxicity, the first to be reported. This mechanism involves the binding of UO 2 ²+ to pyrroloquinoline quinone (PQQ), a cofactor present in a number of bacterial dehydrogenase enzymes. Based on the specific binding mode of UO 2 ²+ to PQQ, it is hypothesized that the present work has direct implications for UO 2 ²+ inhibition of flavoproteins, potentially extending the application of the findings of this work to eukaryotic systems. Recent trends suggest that U-related activity will increase in the near future, and therefore understanding fundamental interactions between UO 2 ²+ and living systems is both an environmental and human health imperative.en
dc.publisherMontana State University - Bozeman, College of Engineeringen
dc.subject.lcshPQQ (Biochemistry)en
dc.titleMolecular aspects of uranium toxicity : speciation and physiological targetingen
dc.rights.holderCopyright 2009 by Michael Robert VanEngelenen
thesis.catalog.ckey1509301en, Graduate Committee: Ross Carlson; Matthew Fieldsen & Biological Engineering.en

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