Show simple item record

dc.contributor.advisorChairperson, Graduate Committee: Mark J. Young.en
dc.contributor.authorWirth, Jennifer Fulton.en
dc.date.accessioned2013-06-25T18:36:52Z
dc.date.available2013-06-25T18:36:52Z
dc.date.issued2011en
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/2564
dc.description.abstractViruses are the most abundant biological entity on earth, and virus-host interactions are one of the most important factors shaping microbial populations (Suttle, 2007b). The study of both the cellular and viral members of the domain Archaea is a relatively new field. Thus, the viruses (and their cellular hosts) of Archaea are poorly understood as compared to viruses of Bacteria and Eukarya. This work has sought to expand our understanding of archaeal viruses by two general approaches. The first is by developing and implementing the use of a genetic system for a crenarchaeal virus, Sulfolobus turreted icosahedral virus (STIV), isolated from a hot (82°C) acidic (pH 2.2) pool in Yellowstone National Park, USA. The second approach has been to look at viral communities and their interactions with their cellular hosts in natural environments. We have developed a genetic tool, an infectious clone for STIV, which has allowed for genetic analysis of this virus. A number of viral genes have been knocked out, and their functions investigated using this tool. We have determined that at least three viral genes, A197, B345 and C381, are required for viral replication, while one gene, B116, is not essential. Work continues investigating function for other STIV genes as well as specific interactions with its host, Sulfolobus solfataricus. We have performed total community sequencing (metagenomics) for both the cellular and viral populations of several hot springs in Yellowstone National Park. We have been able to assemble a near full-length putative novel viral genome from one of these sites. We have also performed an in depth analysis of the function of a newly described bacterial and archaeal adaptive immune system (CRISPR/Cas) in a natural environment. This study has provided insights into the function of this immune system in a complex nutrient limited environment, which would not have been observed by studying cultured isolates in a laboratory.en
dc.language.isoengen
dc.publisherMontana State University - Bozeman, College of Letters & Scienceen
dc.subject.lcshArchaebacteria.en
dc.subject.lcshHost-virus relationships.en
dc.titleArchaeal host virus interactions
dc.typeDissertation
dc.rights.holderCopyright Jennifer Fulton Wirth 2011en
thesis.catalog.ckey1926759en
thesis.degree.committeemembersMembers, Graduate Committee: Martin Teintze; Mensur Dlakic; Michael Franklin; Trevor Douglas; Richard Wolffen
thesis.degree.departmentMicrobiology & Immunology.en
thesis.degree.genreDissertationen
thesis.degree.namePhDen
thesis.format.extentfirstpage1en
thesis.format.extentlastpage175en
mus.identifier.categoryLife Sciences & Earth Sciences
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record