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dc.contributor.advisorChairperson, Graduate Committee: Brent M. Peytonen
dc.contributor.authorPopovitch, Ari Elizabethen
dc.date.accessioned2013-10-08T16:26:02Z
dc.date.available2013-10-08T16:26:02Z
dc.date.issued2012en
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/2876en
dc.description.abstractThermus thermophilus ST and S42 were isolated from high pH (9) and temperature (70°C) hot springs in the Alvord Desert, Oregon and the Heart Lake Geyser Basin in Yellowstone National Park, Wyoming, respectively. The two strains exhibited lignin degrading potential at pH 9 and 70°C, due to their ability to utilize the lignocellulose degradation products kraft lignin, ferulic acid, cinnamic acid, and p-coumaric acid for growth. Growth on the soluble fraction of alkaline pretreated lignocellulose sources, corn stover, corn cob and lodgepole pine was evaluated. The two isolates grew to higher cell yields due of the presence of kraft lignin, corn stover and lodge pole pine when supplemented with glucose. Dye decolorizing activity was confirmed with Remazol Brilliant Blue R (RBBR), an industrial dye and lignin analog compound. Laccase mediated 2,2' azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) activity was observed for both isolates when 0.05 mM CuSO 4 was supplemented to the growth medium. Ligninolytic bacteria capable of growth at pH 9 and 70°C are potentially useful with alkaline lignocellulose pretreatment methods to depolymerize and remove lignin prior to the production of second generation biofuel.en
dc.language.isoenen
dc.publisherMontana State University - Bozeman, College of Engineeringen
dc.subject.lcshThermophilic bacteriaen
dc.subject.lcshLigninen
dc.subject.lcshBiodegradationen
dc.titleIsolation and characterization of thermostable alkaline bacteria with ligninolytic potentialen
dc.typeThesisen
dc.rights.holderCopyright 2012 by Ari Elizabeth Popovitchen
thesis.catalog.ckey2171863en
thesis.degree.committeemembersMembers, Graduate Committee: Abigail Richards; Brady Leeen
thesis.degree.departmentChemical & Biological Engineering.en
thesis.degree.genreThesisen
thesis.degree.nameMSen
thesis.format.extentfirstpage1en
thesis.format.extentlastpage77en


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