Biogeochemical gradients and energetics in geothermal systems of Yellowstone National Park
Ackerman, Galena Gene
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The fate and behavior of redox-active chemical species in geothermal systems is linked with the metabolic processes of chemotrophic thermophilic microorganisms. The major goal of the current work was to perform a thorough geochemical analysis of redox active species in geothermal outflow channels, and utilize these measurements to quantify the Gibbs free energy (?Grxn) values for numerous oxidation-reduction reactions that represent potential chemolithotrophic metabolisms. Insights gained from energetic analyses can be used to structure hypotheses regarding novel microbial metabolisms and to guide cultivation strategies for isolating relevant microorganisms. A comprehensive suite of geochemical parameters, including major ions, trace elements, redox-active species and dissolved gases, were analyzed and monitored in vertical transects of 11 geothermal outflow channels in Yellowstone National Park from 2003-2005. The geothermal springs chosen for this study contained strikingly different aqueous and solid-phase geochemistry. These systems exhibited a wide range of conditions, including ranges in pH (2.7 to 7.0), temperature (60 oC to 92 oC), Cl- (0.01 to 23 mM)), SO42- (0.4 to 7.5 mM), NH4+ (0.02 to 5.7 mM), CO2 (aq) (0.1 to 4.5 mM), Fe (0.2 to 230 uM), and As (0.03 to 130 uM)The predominant changes in geochemistry occurring within geothermal outflow channels were consistently related to the dynamics of air-water gas exchange. In all springs studied, dissolved gases including H2, CO2, CH4 and H2S decreased down gradient of geothermal discharge, while O2 ingassing resulted in increases in dissolved O2. Calculated free energy (?Grxn) values for balanced oxidation-reduction reactions suggest that numerous electron donor/acceptor combinations are exergonic in these systems. Reactions where H2, CH4, H2S, S0, AsIII, FeII and or NH4 serve as electron donors were all significantly exergonic (< -30 kJ mole-1 electron at all sites) when O2 was the electron acceptor, even when O2 levels were at the detection limit (3uM). The geothermal systems included in this study all exhibited significant changes in microbial population distribution from near source-water conditions to sediments lining the outflow channels, consistent with the hypothesis that geochemical gradients and temperature correlate with microbial population distribution.