Scholarly Work - Chemistry & Biochemistry
Recent Submissions
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Distinct properties underlie flavin-based electron bifurcation in a novel electron transfer flavoprotein FixAB from Rhodopseudomonas palustris
(2018-02)A newly recognized third fundamental mechanism of energy conservation in biology, electron bifurcation, uses free energy from exergonic redox reactions to drive endergonic redox reactions. Flavin-based electron bifurcation ... -
Coenzyme M biosynthesis in bacteria involves phosphate elimination by a functionally distinct member of the aspartase/fumarase superfamily
(2018-04)For nearly 30 years, coenzyme M (CoM) was assumed to be present solely in methanogenic archaea. In the late 1990s, CoM was reported to play a role in bacterial propene metabolism, but no biosynthetic pathway for CoM has ... -
Decarboxylation involving a ferryl, propionate, and a tyrosyl group in a radical relay yields heme b
(2018-03)The H2O2-dependent oxidative decarboxylation of coproheme III is the final step in the biosynthesis of heme<em> b</em> in many microbes. However, the coproheme decarboxylase reaction mechanism is unclear. The structure of ... -
Semiochemicals to enhance herbivory by Diorhabda carinulata aggregations in saltcedar (Tamarix spp.) infestations
(2018-01)BACKGROUND: Semiochemicals for monitoring, attracting or repelling pest and beneficial organisms are increasingly deployed in agricultural and forest systems for pest management activities. However, the use of aggregation ... -
H/D exchange mass spectrometry and statistical coupling analysis reveal a role for allostery in a ferredoxin-dependent bifurcating transhydrogenase catalytic cycle
(2018-01)Recent investigations into ferredoxin-dependent transhydrogenases, a class of enzymes responsible for electron transport, have highlighted the biological importance of flavin-based electron bifurcation (FBEB). FBEB generates ... -
Structural Basis for the Mechanism of ATP-Dependent Acetone Carboxylation
(2017-08)Microorganisms use carboxylase enzymes to form new carbon-carbon bonds by introducing carbon dioxide gas (CO2) or its hydrated form, bicarbonate (HCO3 −), into target molecules. Acetone carboxylases (ACs) catalyze the ... -
Ultrafast Excited-State Deactivation of the Bacterial Pigment Violacein
(2017-08)The photophysical properties of the natural pigment violacein extracted from an Antarctic organism adapted to high exposure levels of UV radiation were measured in a combined steady-state and time-resolved spectroscopic ... -
X-ray crystallographic evidence for the simultaneous presence of axial and rhombic sites in cupredoxins: atomic resolution X-ray crystal structure analysis of pseudoazurin and DFT modelling
(2016-09)Crystal structure refinement of pseudoazurin from Achromobacter cycloclastes (AcPAz) was carried out at atomic (1.10 angstrom) resolution. The copper ion was localized in two positions at the metal binding site of AcPAz. ... -
Defining Electron Bifurcation in the Electron Transferring Flavoprotein Family
(2017-11)Electron bifurcation is the coupling of exergonic and endergonic redox reactions to simultaneously generate (or utilize) low and high potential electrons. It is the third recognized form of energy conservation in biology ... -
Structural Basis for the Mechanism of ATP-Dependent Acetone Carboxylation
(2017-08)Microorganisms use carboxylase enzymes to form new carbon-carbon bonds by introducing carbon dioxide gas (CO2) or its hydrated form, bicarbonate (HCO3-), into target molecules. Acetone carboxylases (ACs) catalyze the ... -
Unraveling the interactions of the physiological reductant flavodoxin with the different conformations of the Fe protein in the nitrogenase cycle
(2017-08)Nitrogenase reduces dinitrogen (N2) to ammonia in biological nitrogen fixation. The nitrogenase Fe protein cycle involves a transient association between the reduced, MgATP-bound Fe protein and the MoFe protein and includes ... -
Preparation and Evaluation of Potent PentafluorosulfanylSubstituted Anti-Tuberculosis Compounds
(2017-07)The global fight to stop tuberculosis (TB) remains a great challenge, particularly with the increase in drug-resistant strains and a lack of funding to support the development of new treatments. To bolster a precarious ... -
Indirect Interspecies Regulation: Transcriptional and Physiological Responses of a Cyanobacterium to Heterotrophic Partnership
(2017-03)The mechanisms by which microbes interact in communities remain poorly understood. Here, we interrogated specific interactions between photoautotrophic and heterotrophic members of a model consortium to infer mechanisms ... -
Differences in amino acid catabolism by gut microbes with/without prebiotics inclusion in GDDY-based diet affect feed utilization in rainbow trout
(2017-09)There is the need to enhance feed efficiency and growth of rainbow trout to reduce production costs of cultured fish. This study conducted a 3 × 4 factorial experiment with three graded levels of grain distiller dried yeast ... -
Proteomic and biochemical assays of glutathione-related proteins in susceptible and multiple herbicide resistant Avena fatua L.
(2017-06)Extensive herbicide usage has led to the evolution of resistant weed populations that cause substantial crop yield losses and increase production costs. The multiple herbicide resistant (MHR) Avena fatua L. populations ... -
Thermal stability as a determinant of AAV serotype identity
(2017-09)Currently, there are over 150 ongoing clinical trials utilizing adeno-associated viruses (AAVs) to target various genetic diseases, including hemophilia (AAV2 and AAV8), congenital heart failure (AAV1 and AAV6), cystic ... -
Multi-level Omics Analysis Provides Insight to the Ignicoccus hospitalis-Nanoarchaeum equitans Association
(2017-09)BACKGROUND Studies of interspecies interactions are inherently difficult due to the complex mechanisms which enable these relationships. A model system for studying interspecies interactions is the marine hyperthermophiles ... -
Blue-light induced accumulation of reactive oxygen species is a consequence of the Drosophila cryptochrome photocycle
(2017-03)Cryptochromes are evolutionarily conserved blue-light absorbing flavoproteins which participate in many important cellular processes including in entrainment of the circadian clock in plants, Drosophila and humans. Drosophila ... -
The Quantum Biology of Reactive Oxygen Species Partitioning Impacts Cellular Bioenergetics
(2016-12)Quantum biology is the study of quantum effects on biochemical mechanisms and biological function. We show that the biological production of reactive oxygen species (ROS) in live cells can be influenced by coherent electron ... -
Imaging theory of structured pump-probe microscopy
(2016-09)With sub-micron spatial resolution and femtosecond temporal resolution, pump probe microscopy provides a powerful spectroscopic probe of complex electronic environments in bulk and nanoscale materials. However, the electronic ...
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