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dc.contributor.authorBernstein, Hans C.
dc.contributor.authorKonopka, Allan
dc.contributor.authorMelnicki, Matthew R.
dc.contributor.authorHill, Eric A.
dc.contributor.authorKucek, Leo A.
dc.contributor.authorZhang, Shuyi
dc.contributor.authorShen, Gaozhong
dc.contributor.authorBryant, Donald A.
dc.contributor.authorBeliaev, Alexander S.
dc.identifier.citationBernstein, Hans C., Allan Konopka, Matthew R. Melnicki, Eric A. Hill, Leo A. Kucek, Shuyi Zhang, Gaozhong Shen, Donald A. Bryant, and Alexander S. Beliaev. "Effect of mono-and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002." Frontiers in Microbiology 5 (September 19, 2014). doi:10.3389/fmicb.2014.00488.en_US
dc.description.abstractSynechococcus sp. PCC 7002 was grown to steady state in optically thin turbidostat cultures under conditions for which light quantity and quality was systematically varied by modulating the output of narrow-band LEDs. Cells were provided photons absorbed primarily by chlorophyll (680 nm) or phycocyanin (630 nm) as the organism was subjected to four distinct mono- and dichromatic regimes. During cultivation with dichromatic light, growth rates were generally proportional to the total incident irradiance at values <275 2 μmol photons m− · s−1 and were not affected by the ratio of 630:680 nm wavelengths. Notably, under monochromatic light conditions, cultures exhibited similar growth rates only when they were irradiated with 630 nm light; cultures irradiated with only 680 nm light grew at rates that were 60–70% of those under other light quality regimes at equivalent irradiances. The functionality of photosystem II and associated processes such as maximum rate of photosynthetic electron transport, rate of cyclic electron flow, and rate of dark respiration generally increased as a function of growth rate. Nonetheless, some of the photophysiological parameters measured here displayed distinct patterns with respect to growth rate of cultures adapted to a single wavelength including phycobiliprotein content, which increased under severely light-limited growth conditions. Additionally, the ratio of photosystem II to photosystem I increased ∼40% over the range of growth rates, although cells grown with 680 nm light only had the highest ratios. These results suggest the presence of effective mechanisms which allow acclimation of Synechococcus sp. PCC 7002 acclimation to different irradiance conditions.en_US
dc.description.sponsorshipThe research was supported by the Genomic Science Program (GSP), Office of Biological and Environmental Research (BER), U.S. Department of Energy (DOE), and is a contribution of the Pacific Northwest National Laboratory (PNNL) Biofuels Scientific Focus Area (BSFA). Hans C. Bernstein is grateful for the support of the Laboratory Research and Development Program and Linus Pauling Distinguished Post-doctoral Fellowship program at PNNL. Support for Gaozhong Shen and Shuyi Zhang was partially provided by grant MCB-1021725 from the National Science Foundation to Donald A. Bryant. The authors wish to acknowledge Victoria Work who assisted with the protein concentration measurements. PNNL is operated for the DOE by Battelle Memorial Institute under Contract DE-AC05-76RLO 1830.en_US
dc.publisherFrontiers Research Foundation (OA)en_US
dc.titleEffect of mono-and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002en_US
mus.citation.journaltitleFrontiers in Microbiologyen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agriculture
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupThermal Biology Institute.
mus.contributor.orcidBernstein, Hans C.|0000-0003-2913-7708en_US

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