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dc.contributor.authorWu, Jiahui
dc.contributor.authorAbdelfattah, Ahmed S.
dc.contributor.authorMiraucourt, Loïs S.
dc.contributor.authorKutsarova, Elena
dc.contributor.authorRuangkittisakul, Araya
dc.contributor.authorZhou, Hang
dc.contributor.authorBallanyi, Klaus
dc.contributor.authorWicks, Geoffrey
dc.contributor.authorDrobizhev, Mikhail
dc.contributor.authorRebane, Aleksander
dc.contributor.authorRuthazer, Edward S.
dc.contributor.authorCampbell, Robert E.
dc.identifier.citationWu, Jiahui, Ahmed S. Abdelfattah, Loïs S. Miraucourt, Elena Kutsarova, Araya Ruangkittisakul, Hang Zhou, Klaus Ballanyi et al. "A long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imaging." Nature Communications 5 (2014). Nature Communications 5, Article number: 5262
dc.descriptionCC BY 4.0 license (
dc.description.abstractThe introduction of calcium ion (Ca2+) indicators based on red fluorescent proteins (RFPs) has created new opportunities for multicolour visualization of intracellular Ca2+ dynamics. However, one drawback of these indicators is that they have optimal two-photon excitation outside the near-infrared window (650–1,000 nm) where tissue is most transparent to light. To address this shortcoming, we developed a long Stokes shift ​RFP-based Ca2+ indicator, REX-GECO1, with optimal two-photon excitation at <1,000 nm. REX-GECO1 fluoresces at 585 nm when excited at 480 nm or 910 nm by a one- or two-photon process, respectively. We demonstrate that REX-GECO1 can be used as either a ratiometric or intensiometric Ca2+ indicator in organotypic hippocampal slice cultures (one- and two-photon) and the visual system of albino tadpoles (two-photon). Furthermore, we demonstrate single excitation wavelength two-colour Ca2+ and ​glutamate imaging in organotypic cultures.en_US
dc.publisherNature Publishing Groupen_US
dc.titleA long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imagingen_US
mus.citation.journaltitleNature Communicationsen_US
mus.identifier.categoryPhysics & Mathematicsen_US
mus.relation.collegeCollege of Letters & Science
mus.relation.collegeCollege of Letters & Sciencesen_US
mus.relation.universityMontana State University - Bozemanen_US

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