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dc.contributor.authorWu, Jiahui
dc.contributor.authorAbdelfattah, Ahmed S.
dc.contributor.authorMiraucourt, Loïs S.
dc.contributor.authorKutsarova, Elena
dc.contributor.authorRuangkittisakul, Araya
dc.contributor.authorZhou, Hang
dc.contributor.authorBallanyi, Klaus
dc.contributor.authorWicks, Geoffrey
dc.contributor.authorDrobizhev, Mikhail
dc.contributor.authorRebane, Aleksander
dc.contributor.authorRuthazer, Edward S.
dc.contributor.authorCampbell, Robert E.
dc.date.accessioned2014-12-10T17:49:53Z
dc.date.available2014-12-10T17:49:53Z
dc.date.issued2014-10
dc.identifier.citationWu, Jiahui, Ahmed S. Abdelfattah, Loïs S. Miraucourt, Elena Kutsarova, Araya Ruangkittisakul, Hang Zhou, Klaus Ballanyi et al. "A long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imaging." Nature Communications 5 (2014). Nature Communications 5, Article number: 5262 http://dx.doi.org/10.1038/ncomms6262en_US
dc.identifier.issn2041-1723
dc.identifier.urihttp://dx.doi.org/10.1038/ncomms6262
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/8749
dc.descriptionCC BY 4.0 license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.description.abstractThe introduction of calcium ion (Ca2+) indicators based on red fluorescent proteins (RFPs) has created new opportunities for multicolour visualization of intracellular Ca2+ dynamics. However, one drawback of these indicators is that they have optimal two-photon excitation outside the near-infrared window (650–1,000 nm) where tissue is most transparent to light. To address this shortcoming, we developed a long Stokes shift ​RFP-based Ca2+ indicator, REX-GECO1, with optimal two-photon excitation at <1,000 nm. REX-GECO1 fluoresces at 585 nm when excited at 480 nm or 910 nm by a one- or two-photon process, respectively. We demonstrate that REX-GECO1 can be used as either a ratiometric or intensiometric Ca2+ indicator in organotypic hippocampal slice cultures (one- and two-photon) and the visual system of albino tadpoles (two-photon). Furthermore, we demonstrate single excitation wavelength two-colour Ca2+ and ​glutamate imaging in organotypic cultures.en_US
dc.publisherNature Publishing Groupen_US
dc.subjectNeursciencesen_US
dc.subjectBiologyen_US
dc.titleA long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imagingen_US
dc.typeArticleen_US
mus.citation.journaltitleNature Communicationsen_US
mus.citation.volume5en_US
mus.identifier.categoryPhysics & Mathematicsen_US
mus.identifier.doi10.1038/ncomms6262en_US
mus.relation.collegeCollege of Letters & Science
mus.relation.collegeCollege of Letters & Sciencesen_US
mus.relation.departmentPhysics.en_US
mus.relation.universityMontana State University - Bozemanen_US


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