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dc.contributor.authorLudwig, M.
dc.contributor.authorBryant, Donald A.
dc.date.accessioned2015-02-17T18:54:53Z
dc.date.available2015-02-17T18:54:53Z
dc.date.issued2011-03
dc.identifier.citationLudwig, M. and Bryant, D. A. 2011. Transcription profiling of the cyanobacterium Synechococcus sp. PCC 7002 using high-throughput cDNA sequencing. Front. Microbio. 2:41.en_US
dc.identifier.issn1664-302X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/8855
dc.description.abstractThe genome of the unicellular, euryhaline cyanobacterium Synechococcus sp. PCC 7002 encodes about 3200 proteins. Transcripts were detected for nearly all annotated open reading frames by a global transcriptomic analysis by Next-Generation (SOLiD™) sequencing of cDNA. In the cDNA samples sequenced, ∼90% of the mapped sequences were derived from the 16S and 23S ribosomal RNAs and ∼10% of the sequences were derived from mRNAs. In cells grown photoautotrophically under standard conditions [38°C, 1% (v/v) CO2 in air, 250 μmol photons m−2 s−1], the highest transcript levels (up to 2% of the total mRNA for the most abundantly transcribed genes; e.g., cpcAB, psbA, psaA) were generally derived from genes encoding structural components of the photosynthetic apparatus. High-light exposure for 1 h caused changes in transcript levels for genes encoding proteins of the photosynthetic apparatus, Type-1 NADH dehydrogenase complex and ATP synthase, whereas dark incubation for 1 h resulted in a global decrease in transcript levels for photosynthesis-related genes and an increase in transcript levels for genes involved in carbohydrate degradation. Transcript levels for pyruvate kinase and the pyruvate dehydrogenase complex decreased sharply in cells incubated in the dark. Under dark anoxic (fermentative) conditions, transcript changes indicated a global decrease in transcripts for respiratory proteins and suggested that cells employ an alternative phosphoenolpyruvate degradation pathway via phosphoenolpyruvate synthase (ppsA) and the pyruvate:ferredoxin oxidoreductase (nifJ). Finally, the data suggested that an apparent operon involved in tetrapyrrole biosynthesis and fatty acid desaturation, acsF2–ho2–hemN2–desF, may be regulated by oxygen concentration.en_US
dc.subjectBiochemistryen_US
dc.subjectMicrobiologyen_US
dc.titleTranscription profiling of the cyanobacterium Synechococcus sp. PCC 7002 using high-throughput cDNA sequencingen_US
dc.typeArticleen_US
mus.citation.extentfirstpage2en_US
mus.citation.extentlastpage41en_US
mus.citation.journaltitleFrontiers in Microbiologyen_US
mus.citation.volume2en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.3389/fmicb.2011.00041en_US
mus.relation.collegeCollege of Agriculture
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupThermal Biology Institute.


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