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dc.contributor.authorTherien, Jesse B.
dc.contributor.authorZadvornyy, Oleg A.
dc.contributor.authorPosewitz, Matthew C.
dc.contributor.authorBryant, Donald A.
dc.contributor.authorPeters, John W.
dc.identifier.citationTherien, J. B., Zadvornyy, O., Posewitz, M. C., Bryant, D. A. and Peters, J. W. (2014) Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate- encapsulated strains of Synechococcus sp. PCC 7002. Biotech. Biofuels, in press.en_US
dc.description.abstractBackground: The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC 7002. Results: Optimal growth conditions for co-cultivation of C. reinhardtii with wild-type and mutant strains of Synechococcus sp. 7002 were established. In co-culture, acetate produced by a glycogen synthase knockout mutant of Synechococcus sp. PCC 7002 was able to support the growth of a lipid-accumulating mutant strain of C. reinhardtii defective in starch production. Encapsulation of Synechococcus sp. PCC 7002 using an alginate matrix was successfully employed in co-cultures to limit growth and maintain the stability. Conclusions: The ability of immobilized strains of the cyanobacterium Synechococcus sp. PCC 7002 to produce acetate at a level adequate to support the growth of lipid-accumulating strains of C. reinhartdii offers a potentially practical, photosynthetic alternative to providing exogenous acetate into growth media.en_US
dc.subjectAlternative energyen_US
dc.titleGrowth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains ofSynechococcus sp. PCC 7002en_US
mus.citation.journaltitleBiotechnology for Biofuelsen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Agriculture
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupThermal Biology Institute.
mus.contributor.orcidPeters, John W.|0000-0001-9117-9568en_US
mus.contributor.orcidZadvornyy, Oleg A.|0000-0002-5374-6856en_US

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