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dc.contributor.authorJohnson, Greg
dc.contributor.authorBahnson, C. S.
dc.contributor.authorCochrane, Z. N.
dc.contributor.authorIshii, P.
dc.contributor.authorHokit, D. G.
dc.contributor.authorPlummer, P. J.
dc.contributor.authorBartholomay, L. C.
dc.contributor.authorBlitvich, B. J.
dc.date.accessioned2015-03-17T16:11:14Z
dc.date.available2015-03-17T16:11:14Z
dc.date.issued2014-02
dc.identifier.citationJohnson, Gregory D., Charlie S. Bahnson, Patricia Ishii, Zachary N. Cochrane, D. Grant Hokit, Paul J. Plummer, Lyric C. Bartholomay, and Bradley J. Blitvich. Monitoring sheep and Culicoides midges in Montana for evidence of Bunyamwera serogroup virus infection. Veterinary Record Open. Pages e000071. https://dx.doi.org/10.1136/vetreco-2014-000071en_US
dc.identifier.issn2052-6113
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/8933
dc.description.abstractIntroduction: A serological and entomological investigation was performed to monitor for potential Bunyamwera (BUN) serogroup virus activity in Montana. Results: To facilitate the serological investigation, sera were collected from 104 sheep in 2013 and 2014 and assayed by plaque reduction neutralization test using all six BUN serogroup viruses known to occur in the United States: Cache Valley virus (CVV), Lokern virus (LOKV), Main Drain virus (MDV), Northway virus, Potosi virus and Tensaw virus. BUN serogroup virus-specific antibodies were detected in 41 (39%) sheep. Of these, three were seropositive for MDV, one was seropositive for CVV, one was seropositive for LOKV and 36 had antibodies to an undetermined BUN serogroup virus. Additionally, 30,606 Culicoides sonorensis were collected in 2013 using Centers for Disease Control and Prevention (CDC) light traps and assayed for cytopathic virus by virus isolation in African Green Monkey kidney (Vero) cells. All midges were negative. Almost one-third of the midges were further tested by reverse transcription-polymerase chain reaction using BUN serogroup virus-reactive primers and all were negative. Conclusions: We provide evidence of BUN serogroup virus infection in sheep but not C. sonorensis in Montana in 2013-2014. This study also provides the first evidence of CVV, MDV and LOKV activity in Montana.en_US
dc.subjectAnimal diseasesen_US
dc.subjectVirologyen_US
dc.titleMonitoring sheep and Culicoides midges in Montana for evidence of Bunyamwera serogroup virus infectionen_US
dc.typeArticleen_US
mus.citation.extentfirstpagee000071en_US
mus.citation.extentlastpagee000071en_US
mus.citation.issue1en_US
mus.citation.journaltitleVeterinary Record Openen_US
mus.citation.volume1en_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1136/vetreco-2014-000071en_US
mus.relation.collegeCollege of Agriculture
mus.relation.departmentAnimal & Range Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCentral Ag Research Center.


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