Show simple item record

dc.contributor.advisorChairperson, Graduate Committee: Deborah Keilen
dc.contributor.authorMurphy, Lacey Marieen
dc.coverage.spatialNellis Dunes Recreational Area (Nev.)en
dc.date.accessioned2016-01-03T16:48:34Z
dc.date.available2016-01-03T16:48:34Z
dc.date.issued2015en
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9069
dc.description.abstractThe Nellis Dunes Recreational Area is a popular off road vehicle site in Clark County, Nevada comprised of dust containing high levels of naturally occurring heavy metals: aluminum, arsenic, cesium, chromium, cobalt, copper, iron, lead, magnesium, manganese, strontium, uranium, vanadium, and zinc. A human health risk assessment and toxicological study to estimate the risk for those who visit the area were conducted. Dust samples from throughout the area were collected and grouped into seven "combination units" (CBN) based on composition and emissivity. Clinical chemistry and hematological changes observed in mice from a sub-acute, dose-dependent, inhalation model were analyzed. Epigenetic markers, specifically 5-methylcytosine, were evaluated in genes known to regulate antibody production and secretion to elucidate a possible mode of action for antibody suppression observed in the murine model. Clinical chemistry and/or hematological changes were observed in each CBN unit, while no change was observed in 5-methylcytosine for PRDM1, PAX5, and XBP-1 transcription factor genes regulating the B-Cell to plasma cell differentiation pathway and antibody production. Exposing B6C3F1 mice to 100 mg/kg dust from NDRA did not have effects on the methylation of CpG islands located in promoter regions of PRDM1 or PAX5. Clinical chemistries and hematology varied with the differing characterizations of each CBN unit, though not all results were dose-responsive.en
dc.language.isoengen
dc.publisherMontana State University - Bozeman, College of Letters & Scienceen
dc.subject.lcshMineral dusts.en
dc.subject.lcshClinical chemistry.en
dc.titleExposure to a complex mixture of metals on geogenic dust : analysis of clinical chemistry, hematology, and epigenetic markersen
dc.typeThesisen
dc.rights.holderCopyright 2015 by Lacey Marie Murphy.en
thesis.catalog.ckey2759033en
thesis.degree.committeemembersMembers, Graduate Committee: Deborah Keil (chairperson); Sandra Halonen; Robert K. D. Peterson; Carolyn Banister.en
thesis.degree.departmentMicrobiology & Immunology.en
thesis.degree.genreThesisen
thesis.degree.nameMSen
thesis.format.extentfirstpage1en
thesis.format.extentlastpage136en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record