Scholarly Work - Center for Biofilm Engineering
Recent Submissions
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Single-Cell Infection of Influenza A Virus Using Drop-Based Microfluidics
(American Society for Microbiology, 2022-10)Drop-based microfluidics has revolutionized single-cell studies and can be applied toward analyzing tens of thousands to millions of single cells and their products contained within picoliter-sized drops. Drop-based ... -
Hand hygiene product use by food employees in casual dining and quick-service restaurants
(Elsevier BV, 2023-02)Hand hygiene product usage characteristics by food employees when hand sanitizers are made available are not well understood. To investigate hand hygiene product usage in casual dining and quick-service restaurants, we ... -
Mitigation and use of biofilms in space for the benefit of human space exploration
(Elsevier BV, 2023-12)Biofilms are self-organized communities of microorganisms that are encased in an extracellular polymeric matrix and often found attached to surfaces. Biofilms are widely present on Earth, often found in diverse and sometimes ... -
The biofilm life cycle: expanding the conceptual model of biofilm formation
(Springer Science and Business Media LLC, 2022-10)Bacterial biofilms are often defined as communities of surface-attached bacteria and are typically depicted with a classic mushroom-shaped structure characteristic of Pseudomonas aeruginosa. However, it has become evident ... -
Imaging and plate counting to quantify the effect of an antimicrobial: A case study of a photo-activated chlorine dioxide treatment
(Oxford University Press, 2022-12)Aim. To assess removal versus kill efficacies of antimicrobial treatments against thick biofilms with statistical confidence. Methods and results. A photo‐activated chlorine dioxide treatment (Photo ClO2) was tested in two ... -
Interactions of microorganisms within a urinary catheter polymicrobial biofilm model
(Wiley, 2022-09)Biofilms are often polymicrobial in nature, which can impact their behavior and overall structure, often resulting in an increase in biomass and enhanced antimicrobial resistance. Using plate counts and locked nucleic ... -
The impact of automated hand hygiene monitoring with and without complementary improvement strategies on performance rates
(Cambridge University Press, 2022-08)Objective: To determine how engagement of the hospital and/or vendor with performance improvement strategies combined with an automated hand hygiene monitoring system (AHHMS) influence hand hygiene (HH) performance rates. ... -
Efficacy of Common Antiseptic Solutions Against Clinically Relevant Planktonic Microorganisms
(SLACK, Inc., 2022-03)Prosthetic joint infections (PJIs) are among the most devastating complications after joint replacement. There is limited evidence regarding the efficacy of different antiseptic solutions in reducing planktonic microorganism ... -
Search for a Shared Genetic or Biochemical Basis for Biofilm Tolerance to Antibiotics across Bacterial Species
(American Society for Microbiology, 2022-04)Is there a universal genetically programmed defense providing tolerance to antibiotics when bacteria grow as biofilms? A comparison between biofilms of three different bacterial species by transcriptomic and metabolomic ... -
Antimicrobial Coating Efficacy for Prevention of Pseudomonas aeruginosa Biofilm Growth on ISS Water System Materials
(Frontiers Media SA, 2022-04)Biofilms can lead to biofouling, microbially induced corrosion, physical impediment and eventual loss in function of water systems, and other engineered systems. The remoteness and closed environment of the International ... -
Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research
(MyJove Corporation, 2022-04)Biofilm methods consist of four distinct steps: growing the biofilm in a relevant model, treating the mature biofilm, harvesting the biofilm from the surface and disaggregating the clumps, and analyzing the sample. Of the ... -
Noninflammatory comedones have greater diversity in microbiome and are more prone to biofilm formation than inflammatory lesions of acne vulgaris
(Wiley, 2020-12)Background: The ability of C. acnes strains to form biofilms has been correlated with their virulence. Objective: This study examined biofilm and skin microbiota in acne patients in order to understand their role in the ... -
Isolation and Characterization of Lignocellulose-Degrading Geobacillus thermoleovorans from Yellowstone National Park
(American Society for Microbiology, 2022-05)The microbial degradation of lignocellulose in natural ecosystems presents numerous biotechnological opportunities, including biofuel production from agricultural waste and feedstock biomass. To explore the degradation ... -
The importance of understanding the infectious microenvironment
(Elsevier BV, 2022-03)Standard doses of antibiotics do not efficiently treat chronic infections of the soft tissue and bone. In this Personal View, we advocate for improving treatment of these infections by taking the infectious microenvironment ... -
Investigation of Raman Spectroscopic Signatures with Multivariate Statistics: An Approach for Cataloguing Microbial Biosignatures
(Mary Ann Liebert Inc, 2021-09)Spectroscopic instruments are increasingly being implemented in the search for extraterrestrial life. However, microstructural spectral analyses of alien environments could prove difficult without knowledge on the molecular ... -
Potential use of fungal-bacterial co-cultures for the removal of organic pollutants
(Informa UK Limited, 2021-07)Fungi and bacteria coexist in a wide variety of natural and artificial environments which can lead to their association and interaction – ranging from antagonism to cooperation – that can affect the survival, colonization, ... -
Screening of Additive Formulations Enables Off-Chip Drop Reverse Transcription Quantitative Polymerase Chain Reaction of Single Influenza A Virus Genomes
(American Chemical Society, 2021-03)The miniaturization of polymerase chain reaction (PCR) using drop-based microfluidics allows for amplification of single nucleic acids in aqueous picoliter-sized drops. Accurate data collection during PCR requires that ... -
Interlaboratory evaluations of a standardized quantitative test method for determining the bactericidal and tuberculocidal efficacy of antimicrobial substances on hard non-porous surfaces
(Elsevier BV, 2022-04)The development, validation, and use of new quantitative methodologies for testing the effectiveness of antimicrobial products are necessary to meet the regulatory challenges associated with an ever-changing marketplace, ... -
Ureolysis-induced calcium carbonate precipitation (UICP) in the presence of CO2-affected brine: A field demonstration
(Elsevier BV, 2021-07)Biomineralization is an emerging biotechnology for subsurface engineering applications like remediating leaky wellbores. The process relies on ureolysis to induce precipitation of calcium carbonate in undesired flow paths. ... -
Biofilms vs. cities and humans vs. aliens – a tale of reproducibility in biofilms
(Elsevier BV, 2021-06)In recent decades the scientific community has started to appreciate that most microorganisms live in complex 3D structures composed of cells, polysaccharides, and other components such as proteins, extracellular (e)DNA, ...