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dc.contributor.authorAmmons, Mary Cloud B.
dc.contributor.authorMorrissey, Kathryn
dc.contributor.authorTripet, Brian P.
dc.contributor.authorVan Leuvan, James T.
dc.contributor.authorHan, Anne
dc.contributor.authorLazarus, Gerald S.
dc.contributor.authorZenilman, Jonathan M.
dc.contributor.authorStewart, Philip S.
dc.contributor.authorJames, Garth A.
dc.contributor.authorCopie, Valerie
dc.identifier.citationAmmons, Mary Cloud B., Kathryn Morrissey, Brian P. Tripet, James T. Van Leuven, Anne Han, Gerald S. Lazarus, Jonathan M. Zenilman, Philip S. Stewart, Garth A. James, and Valérie Copié. "Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds." PLoS ONE 10, no. 5 (May 2015): e0126735. DOI:
dc.description.abstractChronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in chronic wounds this process seems to become dysregulated at the transition between resolution of inflammation and re-epithelialization. Bioburden in the form of colonizing bacteria is a major contributor to the delayed headlining in chronic wounds such as pressure ulcers. However how the microbiome influences the wound metabolic landscape is unknown. Here, we have used a Systems Biology approach to determine the biochemical associations between the taxonomic and metabolomic profiles of wounds colonized by bacteria. Pressure ulcer biopsies were harvested from primary chronic wounds and bisected into top and bottom sections prior to analysis of microbiome by pyrosequencing and analysis of metabolome using 1H nuclear magnetic resonance (NMR) spectroscopy. Bacterial taxonomy revealed that wounds were colonized predominantly by three main phyla, but differed significantly at the genus level. While taxonomic profiles demonstrated significant variability between wounds, metabolic profiles shared significant similarity based on the depth of the wound biopsy. Biochemical association between taxonomy and metabolic landscape indicated significant wound-to-wound similarity in metabolite enrichment sets and metabolic pathway impacts, especially with regard to amino acid metabolism. To our knowledge, this is the first demonstration of a statistically robust correlation between bacterial colonization and metabolic landscape within the chronic wound environment.en_US
dc.description.sponsorshipNIH grant 3P20GM103394-05S1; NIH grant 1KO1GM103821-01; NIH grant 1RO3AR060995-01A1; NIH award R01GM109452en_US
dc.rightsCC BY 4.0en_US
dc.titleBiochemical association of metabolic profile and microbiome in chronic pressure ulcer woundsen_US
mus.citation.journaltitlePLoS ONEen_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidCopie, Valerie|0000-0002-2778-1463en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US
mus.contributor.orcidAmmons, Mary Cloud B.|0000-0002-9717-0844en_US

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