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dc.contributor.authorConnolly, James M.
dc.contributor.authorJackson, Benjamin
dc.contributor.authorRothman, Adam P.
dc.contributor.authorKlapper, Isaac
dc.contributor.authorGerlach, Robin
dc.date.accessioned2015-12-09T22:23:16Z
dc.date.available2015-12-09T22:23:16Z
dc.date.issued2015-09
dc.identifier.citationConnolly, James M, Benjamin Jackson, Adam P. Rothman, Isaac Klapper, and Robin Gerlach. "Estimation of a biofilm-specific reaction rate: kinetics of bacterial urea hydrolysis in a biofilm." NPJ Biofilms & Microbiomes 1 (September 2015): 15014. DOI:https://dx.doi.org/10.1038/npjbiofilms.2015.14.en_US
dc.identifier.issn2055-5008
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9405
dc.description.abstractBackground/Objectives: Biofilms and specifically urea-hydrolysing biofilms are of interest to the medical community (for example, urinary tract infections), scientists and engineers (for example, microbially induced carbonate precipitation). To appropriately model these systems, biofilm-specific reaction rates are required. A simple method for determining biofilm-specific reaction rates is described and applied to a urea-hydrolysing biofilm. Methods: Biofilms were grown in small silicon tubes and influent and effluent urea concentrations were determined. Immediately after sampling, the tubes were thin sectioned to estimate the biofilm thickness profile along the length of the tube. Urea concentration and biofilm thickness data were used to construct an inverse model for the estimation of the urea hydrolysis rate. Results/Conclusions: It was found that urea hydrolysis in Escherichia coli MJK2 biofilms is well approximated by first-order kinetics between urea concentrations of 0.003 and 0.221 mol/l (0.186 and 13.3 g/l). The first-order rate coefficient (k1) was estimated to be 23.2±6.2 h−1. It was also determined that advection dominated the experimental system rather than diffusion, and that urea hydrolysis within the biofilms was not limited by diffusive transport. Beyond the specific urea-hydrolysing biofilm discussed in this work, the method has the potential for wide application in cases where biofilm-specific rates must be determined.en_US
dc.description.sponsorshipNSF award No. DMS-0934696; U.S. Department of Energy Grant Numbers DE-FG-02-09ER64758, DE-FE0004478, DE-FE0009599, DE-FG02-13ER86571; NSF-IGERT fellowship in Geobiological Systems at Montana State University (DGE-0654336en_US
dc.rightsCC BY 4.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcodeen_US
dc.titleEstimation of a biofilm-specific reaction rate: kinetics of bacterial urea hydrolysis in a biofilmen_US
dc.typeArticleen_US
mus.citation.extentfirstpage15014en_US
mus.citation.journaltitleNPJ Biofilms & Microbiomesen_US
mus.citation.volume1en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1038/npjbiofilms.2015.14en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentMathematical Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage3en_US


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