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dc.contributor.authorXie, Jie
dc.contributor.authorStrobel, Gary A.
dc.contributor.authorFeng, Tao
dc.contributor.authorRen, Huishuang
dc.contributor.authorMends, Morgan Tess
dc.contributor.authorNhou, Zeyang
dc.contributor.authorGeary, Brad
dc.date.accessioned2015-12-28T21:25:51Z
dc.date.available2015-12-28T21:25:51Z
dc.date.issued2015-06
dc.identifier.citationXie, Jie, Gary A. Strobel, Tao Feng, Huishuang Ren, Morgan T. Mends, Zeyang Zhou, and Brad Geary. "An endophytic Coniochaeta velutina producing broad spectrum antimycotics." Journal of Microbiology 53, no. 6 (June 2015): 390-397. DOI:https://dx.doi.org/10.1007/s12275-015-5105-5.en_US
dc.identifier.issn1225-8873
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9466
dc.description.abstractAn endophyte (PC27-5) was isolated from stem tissue of Western hemlock (Tsuga heterophylla) in a Pacific Northwest temperate rainforest. Phylogenetic analyses, based on ITS-5.8S rDNA and 18S rDNA sequence data, combined with cultural and morphological analysis showed that endophyte PC27-5 exhibited all characteristics of a fungus identical to Coniochaeta velutina. Furthermore, wide spectrum antimycotics were produced by this endophyte that were active against such plant pathogens as Sclerotinia sclerotiorum, Pythium ultimum, and Verticillium dahliae and lethal to Phythophthora cinnamomi, Pythium ultimum, and Phytophthora palmivora in plate tests. The bioactive components were purified through organic solvent extraction, followed by silica column chromatography, and finally preparative HPLC. The minimum inhibitory concentration of the active fraction to Pythium ultimum, which was gained from preparative HPLC, was 11 μg/ml. UPLC-HRMS analysis showed there were two similar components in the antimycotic fraction. Their molecular formulae were established as C30H22O11 (compound I) and C30H22O10 (compound II) respectively, and preliminary spectral results indicate that they are anthroquinone glycosides. Other non–biologically active compounds were identified in culture fluids of this fungus by spectral means as emodin and chrysophanol - anthroquinone derivatives. This is the first report that Coniochaeta velutina as an endophyte produces bioactive antifungal components.en_US
dc.titleAn endophytic Coniochaeta velutina producing broad spectrum antimycoticsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage390en_US
mus.citation.extentlastpage397en_US
mus.citation.issue6en_US
mus.citation.journaltitleJournal of Microbiologyen_US
mus.citation.volume53en_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1007/s12275-015-5105-5en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US


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