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dc.contributor.authorZhou, Yang
dc.contributor.authorHanks, Tracey S.
dc.contributor.authorFeng, Wenchao
dc.contributor.authorLi, Jinquan
dc.contributor.authorLiu, Guanghui
dc.contributor.authorLiu, Mengyao
dc.contributor.authorLei, Benfang
dc.date.accessioned2016-01-27T19:29:45Z
dc.date.available2016-01-27T19:29:45Z
dc.date.issued2013-11
dc.identifier.citationZhou, Yang, Tracey S Hanks, Wenchao Feng, Jinquan Li, Guanghui Liu, Mengyao Liu, and Benfang Lei. “ The sagA / Pel Locus Does Not Regulate the Expression of the M Protein of the M1T1 Lineage of Group A Streptococcus .” Virulence 4, no. 8 (November 15, 2013): 698–706. doi:10.4161/viru.26413.en_US
dc.identifier.issn2150-5594
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9524
dc.description.abstractAltered expression of Group A Streptococcus (GAS) virulence factors, including the M protein, can result as a consequence of spontaneous genetic changes that occur during laboratory and animal passage. Occurrence of such secondary mutations during targeted gene deletion could confound the interpretation of effects attributable to the function of the gene being investigated. Contradicting reports on whether the sagA/pel locus regulates the M protein-encoding emm might be due to inconsistent occurrence of mutations unrelated with sagA. This study examined the possibility that altered emm expression observed in association with sagA/pel deletion mutants is artifactual. sagA deletion mutants (MGAS2221ΔsagA) of M1T1 isolate MGAS2221 obtained using liquid broth for GAS growth during the deletion process had diminished emm transcription and no detectable M protein production. In contrast, a ΔsagA mutant of another closely genetically related M1T1 isolate had normal emm expression. The sagB gene does not regulate emm; however, one of three MGAS2221ΔsagB mutants had diminished emm expression. The emm regulator mga was downregulated in these M protein expression-negative strains. These results argue that sagA deletion does not directly cause the downregulation of emm expression. Indeed, two MGAS2221ΔsagA mutants obtained using agar plates for GAS growth during the deletion process both had normal emm expression. We conclude that the sagA/pel locus does not regulate emm expression in the M1T1 lineage and provide a protocol for targeted gene deletion that we find less prone to the generation of mutants exhibiting downregulation in emm expression.en_US
dc.description.sponsorshipThis work was supported in part by grants AI095704, AI097703, and GM103500-09 from the National Institutes of Health, USDA Animal Formula Fund, and the Montana State Agricultural Experimental Station. We thank Dr James Dale at University of Tennessee Health Science Center for providing anti-M protein antisera and Dr Daniel Nelson at University of Maryland for providing PlyC.en_US
dc.titleThe sagA / pel locus does not regulate the expression of the M protein of the M1T1 lineage of group A Streptococcusen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage9en_US
mus.citation.issue8en_US
mus.citation.journaltitleVirulenceen_US
mus.citation.volume4en_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.doi10.4161/viru.26413en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage5en_US


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