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dc.contributor.authorKumar, Vipan
dc.contributor.authorJha, Prashant
dc.identifier.citationKumar, Vipan, and Prashant Jha. "Growth & Reproduction of Glyphosate-Resistant & Susceptible Populations of Kochia scoparia." PLoS One 10, no. 11 (November 2015): e0142675. DOI: .en_US
dc.description.abstractEvolution of glyphosate-resistant kochia is a threat to no-till wheat-fallow and glyphosate-resistant (GR) cropping systems of the US Great Plains. The EPSPS (5-enol-pyruvylshikimate-3-phosphate synthase) gene amplification confers glyphosate resistance in the tested Kochia scoparia (L.) Schrad populations from Montana. Experiments were conducted in spring to fall 2014 (run 1) and summer 2014 to spring 2015 (run 2) to investigate the growth and reproductive traits of the GR vs. glyphosate-susceptible (SUS) populations of K. scoparia and to determine the relationship of EPSPS gene amplification with the level of glyphosate resistance. GR K. scoparia inbred lines (CHES01 and JOP01) exhibited 2 to 14 relative copies of the EPSPS gene compared with the SUS inbred line with only one copy. In the absence of glyphosate, no differences in growth and reproductive parameters were evident between the tested GR and SUS inbred lines, across an intraspecific competition gradient (1 to 170 plants m-2). GR K. scoparia plants with 2 to 4 copies of the EPSPS gene survived the field-use rate (870 g ha-1) of glyphosate, but failed to survive the 4,350 g ha-1 rate of glyphosate (five-times the field-use rate). In contrast, GR plants with 5 to 14 EPSPS gene copies survived the 4,350 g ha-1 of glyphosate. The results from this research indicate that GR K. scoparia with 5 or more EPSPS gene copies will most likely persist in field populations, irrespective of glyphosate selection pressure.en_US
dc.description.sponsorshipMontana Wheat and Barley Committeeen_US
dc.titleGrowth & Reproduction of Glyphosate-Resistant & Susceptible Populations of Kochia scopariaen_US
mus.citation.journaltitlePloS ONEen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentResearch Centers.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.contributor.orcidKumar, Vipan|0000-0002-8301-5878en_US

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