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dc.contributor.authorSpirig, Thomas
dc.contributor.authorMalmirchegini, G. Reza
dc.contributor.authorZhang, Jiang
dc.contributor.authorRobson, Scott A.
dc.contributor.authorSjodt, Megan
dc.contributor.authorLiu, Mengyao
dc.contributor.authorKrishna Kumar, Kaavya
dc.contributor.authorDickson, Claire
dc.contributor.authorGell, David A.
dc.contributor.authorLei, Benfang
dc.contributor.authorLoo, Joseph A.
dc.contributor.authorClubb, Robert T.
dc.date.accessioned2016-02-26T13:43:06Z
dc.date.available2016-02-26T13:43:06Z
dc.date.issued2012-11
dc.identifier.citationSpirig T, Malmirchegini GR, Zhang J, Robson SA, Sjodt M, Liu M, Krishna Kumar K, Dickson CF, Gell DA, Lei B, Loo JA, Clubb RT. 2013. Staphylococcus aureus Uses a Novel Multidomain Receptor to Break Apart Human Hemoglobin and Steal Its Heme. Journal of Biological Chemistry 288(2):1065-1078.en_US
dc.identifier.issn0021-9258
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9603
dc.description.abstractStaphylococcus aureus is a leading cause of life-threatening infections in the United States. It requires iron to grow, which must be actively procured from its host to successfully mount an infection. Heme-iron within hemoglobin (Hb) is the most abundant source of iron in the human body and is captured by S. aureus using two closely related receptors, IsdH and IsdB. Here we demonstrate that each receptor captures heme using two conserved near iron transporter (NEAT) domains that function synergistically. NMR studies of the 39-kDa conserved unit from IsdH (IsdHN2N3, Ala326–Asp660) reveals that it adopts an elongated dumbbell-shaped structure in which its NEAT domains are properly positioned by a helical linker domain, whose three-dimensional structure is determined here in detail. Electrospray ionization mass spectrometry and heme transfer measurements indicate that IsdHN2N3 extracts heme from Hb via an ordered process in which the receptor promotes heme release by inducing steric strain that dissociates the Hb tetramer. Other clinically significant Gram-positive pathogens capture Hb using receptors that contain multiple NEAT domains, suggesting that they use a conserved mechanism.en_US
dc.titleStaphylococcus aureus Uses a Novel Multidomain Receptor to Break Apart Human Hemoglobin and Steal Its Hemeen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1065en_US
mus.citation.extentlastpage1078en_US
mus.citation.issue2en_US
mus.citation.journaltitleJournal of Biological Chemistryen_US
mus.citation.volume288en_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.doi10.1074/jbc.m112.419119en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage5en_US


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