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dc.contributor.authorMelendrez, Melanie C.
dc.contributor.authorBecraft, Eric D.
dc.contributor.authorWood, Jason M.
dc.contributor.authorOlsen, MIllie T.
dc.contributor.authorBryant, Donald A.
dc.contributor.authorHeidelberg, John F.
dc.contributor.authorRusch, Douglas B.
dc.contributor.authorCohan, Frederick M.
dc.contributor.authorWard, David M.
dc.date.accessioned2016-02-29T22:40:39Z
dc.date.available2016-02-29T22:40:39Z
dc.date.issued2016-01
dc.identifier.citationMelendrez, Melanie C. , Eric D. Becraft, Jason M. Wood, Millie T. Olsen, Donald A. Bryant, John F. Heidelberg, Douglas B. Rusch, Frederick M. Cohan, and David M. Ward. "Recombination Does Not Hinder Formation or Detection of Ecological Species of Synechococcus Inhabiting a Hot Spring Cyanobacterial Mat." Frontiers in Microbiology 6, no. 1540 (January 2016). DOI: 10.3389/fmicb.2015.01540.en_US
dc.identifier.issn1664-302X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/9607
dc.descriptionThis paper is the update and product of analyses (both new and what was included in the dissertation) based on chapters 3 and 4 of the 2010 dissertation by the same author "Population genetics of Synehococcus species inhabiting the Mushroom Spring microbial mat, Yellowstone National Park" which discuss bacterial artificial chromosome libraries and cultivation-independent multi-locus sequence analysis.en_US
dc.description.abstractRecent studies of bacterial speciation have claimed to support the biological species concept—that reduced recombination is required for bacterial populations to diverge into species. This conclusion has been reached from the discovery that ecologically distinct clades show lower rates of recombination than that which occurs among closest relatives. However, these previous studies did not attempt to determine whether the more-rapidly recombining close relatives within the clades studied may also have diversified ecologically, without benefit of sexual isolation. Here we have measured the impact of recombination on ecological diversification within and between two ecologically distinct clades (A and B') of Synechococcus in a hot spring microbial mat in Yellowstone National Park, using a cultivation-free, multi-locus approach. Bacterial artificial chromosome (BAC) libraries were constructed from mat samples collected at 60°C and 65°C. Analysis of multiple linked loci near Synechococcus 16S rRNA genes showed little evidence of recombination between the A and B' lineages, but a record of recombination was apparent within each lineage. Recombination and mutation rates within each lineage were of similar magnitude, but recombination had a somewhat greater impact on sequence diversity than mutation, as also seen in many other bacteria and archaea. Despite recombination within the A and B' lineages, there was evidence of ecological diversification within each lineage. The algorithm Ecotype Simulation identified sequence clusters consistent with ecologically distinct populations (ecotypes), and several hypothesized ecotypes were distinct in their habitat associations and in their adaptations to different microenvironments. We conclude that sexual isolation is more likely to follow ecological divergence than to precede it. Thus, an ecology-based model of speciation appears more appropriate than the biological species concept for bacterial and archaeal diversification.en_US
dc.language.isoen_USen_US
dc.publisherFrontiers Mediaen_US
dc.relation.ispartofhttp://scholarworks.montana.edu/xmlui/handle/1/1856en_US
dc.relation.ispartofhttps://scholarworks.montana.edu/xmlui/handle/1/9608
dc.rights© 2016 Melendrez, Becraft, Wood, Olsen, Bryant, Heidelberg, Rusch, Cohan and Ward. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleRecombination Does Not Hinder Formation or Detection of Ecological Species of Synechococcus Inhabiting a Hot Spring Cyanobacterial Maten_US
dc.typeArticleen_US
mus.citation.extentfirstpage1540en_US
mus.citation.extentlastpage1540en_US
mus.citation.journaltitleFrontiers in Microbiologyen_US
mus.citation.volume6en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.3389/fmicb.2015.01540en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage8en_US


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© 2016 Melendrez, Becraft, Wood, Olsen, Bryant, Heidelberg, Rusch, Cohan and Ward. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as © 2016 Melendrez, Becraft, Wood, Olsen, Bryant, Heidelberg, Rusch, Cohan and Ward. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.