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dc.contributor.authorMelendrez, Melanie C.
dc.contributor.authorLange, Rachel K.
dc.contributor.authorCohan, Frederick M.
dc.contributor.authorWard, David M.
dc.identifier.citationMelanie C. Melendrez, Rachel K. Lange, Frederick M. Cohan and David M. Ward. (2011). Influence of Molecular Resolution on Sequence-Based Discovery of Ecological Diversity among Synechococcus Populations in an Alkaline Siliceous Hot Spring Microbial Mat. Applied and Environmental Microbiology. 77 (4) 1359-1367en_US
dc.descriptionThis paper is the update and product of analyses (both new and what was included in the dissertation) based on chapters 3 and 4 of the 2010 dissertation by the same author "Population genetics of Synehococcus species inhabiting the Mushroom Spring microbial mat, Yellowstone National Park" which discuss bacterial artificial chromosome libraries and cultivation-independent multi-locus sequence analysis.en_US
dc.description.abstractPrevious research has shown that sequences of 16S rRNA genes and 16S-23S rRNA internal transcribed spacer regions may not have enough genetic resolution to define all ecologically distinct Synechococcus populations (ecotypes) inhabiting alkaline, siliceous hot spring microbial mats. To achieve higher molecular resolution, we studied sequence variation in three protein-encoding loci sampled by PCR from 60°C and 65°C sites in the Mushroom Spring mat (Yellowstone National Park, WY). Sequences were analyzed using the ecotype simulation (ES) and AdaptML algorithms to identify putative ecotypes. Between 4 and 14 times more putative ecotypes were predicted from variation in protein-encoding locus sequences than from variation in 16S rRNA and 16S-23S rRNA internal transcribed spacer sequences. The number of putative ecotypes predicted depended on the number of sequences sampled and the molecular resolution of the locus. Chao estimates of diversity indicated that few rare ecotypes were missed. Many ecotypes hypothesized by sequence analyses were different in their habitat specificities, suggesting different adaptations to temperature or other parameters that vary along the flow channel.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsCopyright © 2011, American Society for Microbiologyen_US
dc.titleInfluence of Molecular Resolution on Sequence-Based Discovery of Ecological Diversity among Synechococcus Populations in an Alkaline Siliceous Hot Spring Microbial Maten_US
mus.citation.journaltitleApplied and Environmental Microbiologyen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.contributor.orcidMelendrez, Melanie C.|0000-0002-4811-4467en_US

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